Identification of a trypsin-like serine protease from Trichoderma reesei QM9414
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Identification of a trypsin-like serine protease from Trichoderma reesei QM9414. / Dienes, Dóra; Börjesson, Johan; Hägglund, Per; Tjerneld, Folke; Lidén, Gunnar; Réczey, Kati; Stålbrand, Henrik.
In: Enzyme and Microbial Technology, Vol. 40, No. 5, 03.04.2007, p. 1087-1094.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of a trypsin-like serine protease from Trichoderma reesei QM9414
AU - Dienes, Dóra
AU - Börjesson, Johan
AU - Hägglund, Per
AU - Tjerneld, Folke
AU - Lidén, Gunnar
AU - Réczey, Kati
AU - Stålbrand, Henrik
PY - 2007/4/3
Y1 - 2007/4/3
N2 - In the present work the genetically modified Trichoderma reesei strain QM9414 was used to produce full-length Cel7B (endoglucanase I, EGI) under the control of the constitutive Aspergillus nidulans gpdA promoter in the presence of glucose. However, the full-length Cel7B enzyme was found to be truncated to lower molecular weight components in the culture broth. Truncation of recombinant proteins produced in fungi may be due to protease activity. In order to identify major sectreted proteases, protease activity was assessed in culture filtrate of the T. reesei QM9414 recombinant. Zymogram analysis revealed the presence of proteolytic activity corresponding to one protein, which was subsequently purified by a combination of ion exchange and size exclusion chromatography. The protein has a molecular mass of 25 kDa, and an isoelectric point of 7.3. By matching tryptic peptide fragments analyzed by tandem mass spectrometry to fungal proteins available in databases as well as to expressed sequence tag (EST) sequences, and comparing the coded amino acids to full-length amino acid sequences, the purified protein was found to be homologous to several trypsin-like fungal serine proteases, with the highest homology to the protease P27 from Trichoderma harzianum. The purified protein was further characterized using benzoyl-arginyl-p-nitroanilide (BApNA) as substrate. It was found to have maximum activity at pH 8 and 50 °C, with a km-value of 0.3 mM.
AB - In the present work the genetically modified Trichoderma reesei strain QM9414 was used to produce full-length Cel7B (endoglucanase I, EGI) under the control of the constitutive Aspergillus nidulans gpdA promoter in the presence of glucose. However, the full-length Cel7B enzyme was found to be truncated to lower molecular weight components in the culture broth. Truncation of recombinant proteins produced in fungi may be due to protease activity. In order to identify major sectreted proteases, protease activity was assessed in culture filtrate of the T. reesei QM9414 recombinant. Zymogram analysis revealed the presence of proteolytic activity corresponding to one protein, which was subsequently purified by a combination of ion exchange and size exclusion chromatography. The protein has a molecular mass of 25 kDa, and an isoelectric point of 7.3. By matching tryptic peptide fragments analyzed by tandem mass spectrometry to fungal proteins available in databases as well as to expressed sequence tag (EST) sequences, and comparing the coded amino acids to full-length amino acid sequences, the purified protein was found to be homologous to several trypsin-like fungal serine proteases, with the highest homology to the protease P27 from Trichoderma harzianum. The purified protein was further characterized using benzoyl-arginyl-p-nitroanilide (BApNA) as substrate. It was found to have maximum activity at pH 8 and 50 °C, with a km-value of 0.3 mM.
KW - Cel7B (endoglucanase I)
KW - Serine protease
KW - Trichoderma reesei
KW - Trypsin
UR - http://www.scopus.com/inward/record.url?scp=33847617488&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2006.08.013
DO - 10.1016/j.enzmictec.2006.08.013
M3 - Journal article
AN - SCOPUS:33847617488
VL - 40
SP - 1087
EP - 1094
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 5
ER -
ID: 240161281