Identification and complete genome analysis of a novel bovine picornavirus in Japan

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Identification and complete genome analysis of a novel bovine picornavirus in Japan. / Nagai, Makoto; Omatsu, Tsutomu; Aoki, Hiroshi; Kaku, Yoshihiro; Belsham, Graham J; Haga, Kei; Naoi, Yuki; Sano, Kaori; Umetsu, Moeko; Shiokawa, Mai; Tsuchiaka, Shinobu; Furuya, Tetsuya; Okazaki, Sachiko; Katayama, Yukie; Oba, Mami; Shirai, Junsuke; Katayama, Kazuhiko; Mizutani, Tetsuya.

In: Virus Research, Vol. 210, 02.12.2015, p. 205-12.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nagai, M, Omatsu, T, Aoki, H, Kaku, Y, Belsham, GJ, Haga, K, Naoi, Y, Sano, K, Umetsu, M, Shiokawa, M, Tsuchiaka, S, Furuya, T, Okazaki, S, Katayama, Y, Oba, M, Shirai, J, Katayama, K & Mizutani, T 2015, 'Identification and complete genome analysis of a novel bovine picornavirus in Japan', Virus Research, vol. 210, pp. 205-12. https://doi.org/10.1016/j.virusres.2015.08.001

APA

Nagai, M., Omatsu, T., Aoki, H., Kaku, Y., Belsham, G. J., Haga, K., Naoi, Y., Sano, K., Umetsu, M., Shiokawa, M., Tsuchiaka, S., Furuya, T., Okazaki, S., Katayama, Y., Oba, M., Shirai, J., Katayama, K., & Mizutani, T. (2015). Identification and complete genome analysis of a novel bovine picornavirus in Japan. Virus Research, 210, 205-12. https://doi.org/10.1016/j.virusres.2015.08.001

Vancouver

Nagai M, Omatsu T, Aoki H, Kaku Y, Belsham GJ, Haga K et al. Identification and complete genome analysis of a novel bovine picornavirus in Japan. Virus Research. 2015 Dec 2;210:205-12. https://doi.org/10.1016/j.virusres.2015.08.001

Author

Nagai, Makoto ; Omatsu, Tsutomu ; Aoki, Hiroshi ; Kaku, Yoshihiro ; Belsham, Graham J ; Haga, Kei ; Naoi, Yuki ; Sano, Kaori ; Umetsu, Moeko ; Shiokawa, Mai ; Tsuchiaka, Shinobu ; Furuya, Tetsuya ; Okazaki, Sachiko ; Katayama, Yukie ; Oba, Mami ; Shirai, Junsuke ; Katayama, Kazuhiko ; Mizutani, Tetsuya. / Identification and complete genome analysis of a novel bovine picornavirus in Japan. In: Virus Research. 2015 ; Vol. 210. pp. 205-12.

Bibtex

@article{293af6e924ae4ce0abcf570eb831268d,
title = "Identification and complete genome analysis of a novel bovine picornavirus in Japan",
abstract = "We identified novel viruses in feces from cattle with diarrhea collected in 2009 in Hokkaido Prefecture, Japan, by using a metagenomics approach and determined the (near) complete sequences of the virus. Sequence analyses revealed that they had a standard picornavirus genome organization, i.e. 5' untranslated region (UTR) - L- P1 (VP4- VP3- VP2- VP1) - P2 (2A- 2B- 2C) - P3 (3A- 3B- 3C-3D) - 3'UTR- poly(A). They are closely related to other unclassified Chinese picornaviruses; bat picornaviruses group 1-3, feline picornavirus, and canine picornavirus, sharing 45.4-51.4% (P1), 38.0-44.9% (P2), and 49.6-53.3% (P3) amino acid identities, respectively. The phylogenetic analyses and detailed genome characterization showed that they, together with the unclassified Chinese picornaviruses, grouped as a cluster for the P1, 2C, 3CD and VP1 coding regions. These viruses had conserved features (e.g. predicted protein cleavage sites, presence of a leader protein, 2A, 2C, 3C, and 3D functional domains), suggesting they have a common ancestor. Reverse-transcription-PCR assays, using specific primers designed from the 5'UTR sequence of these viruses, showed that 23.0% (20/87) of fecal samples from cattle with diarrhea were positive, indicating the prevalence of these picornavirus in the Japanese cattle population in Hokkaido Prefecture. However, further studies are needed to investigate the pathogenic potential and etiological role of these viruses in cattle. ",
keywords = "Animals, Cattle, Cattle Diseases/epidemiology, Cluster Analysis, Diarrhea/epidemiology, Feces/virology, Gene Order, Genes, Viral, Genome, Viral, Japan/epidemiology, Molecular Sequence Data, Phylogeny, Picornaviridae/classification, Picornaviridae Infections/veterinary, Prevalence, RNA, Viral/genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology",
author = "Makoto Nagai and Tsutomu Omatsu and Hiroshi Aoki and Yoshihiro Kaku and Belsham, {Graham J} and Kei Haga and Yuki Naoi and Kaori Sano and Moeko Umetsu and Mai Shiokawa and Shinobu Tsuchiaka and Tetsuya Furuya and Sachiko Okazaki and Yukie Katayama and Mami Oba and Junsuke Shirai and Kazuhiko Katayama and Tetsuya Mizutani",
note = "Copyright {\textcopyright} 2015 Elsevier B.V. All rights reserved.",
year = "2015",
month = dec,
day = "2",
doi = "10.1016/j.virusres.2015.08.001",
language = "English",
volume = "210",
pages = "205--12",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Identification and complete genome analysis of a novel bovine picornavirus in Japan

AU - Nagai, Makoto

AU - Omatsu, Tsutomu

AU - Aoki, Hiroshi

AU - Kaku, Yoshihiro

AU - Belsham, Graham J

AU - Haga, Kei

AU - Naoi, Yuki

AU - Sano, Kaori

AU - Umetsu, Moeko

AU - Shiokawa, Mai

AU - Tsuchiaka, Shinobu

AU - Furuya, Tetsuya

AU - Okazaki, Sachiko

AU - Katayama, Yukie

AU - Oba, Mami

AU - Shirai, Junsuke

AU - Katayama, Kazuhiko

AU - Mizutani, Tetsuya

N1 - Copyright © 2015 Elsevier B.V. All rights reserved.

PY - 2015/12/2

Y1 - 2015/12/2

N2 - We identified novel viruses in feces from cattle with diarrhea collected in 2009 in Hokkaido Prefecture, Japan, by using a metagenomics approach and determined the (near) complete sequences of the virus. Sequence analyses revealed that they had a standard picornavirus genome organization, i.e. 5' untranslated region (UTR) - L- P1 (VP4- VP3- VP2- VP1) - P2 (2A- 2B- 2C) - P3 (3A- 3B- 3C-3D) - 3'UTR- poly(A). They are closely related to other unclassified Chinese picornaviruses; bat picornaviruses group 1-3, feline picornavirus, and canine picornavirus, sharing 45.4-51.4% (P1), 38.0-44.9% (P2), and 49.6-53.3% (P3) amino acid identities, respectively. The phylogenetic analyses and detailed genome characterization showed that they, together with the unclassified Chinese picornaviruses, grouped as a cluster for the P1, 2C, 3CD and VP1 coding regions. These viruses had conserved features (e.g. predicted protein cleavage sites, presence of a leader protein, 2A, 2C, 3C, and 3D functional domains), suggesting they have a common ancestor. Reverse-transcription-PCR assays, using specific primers designed from the 5'UTR sequence of these viruses, showed that 23.0% (20/87) of fecal samples from cattle with diarrhea were positive, indicating the prevalence of these picornavirus in the Japanese cattle population in Hokkaido Prefecture. However, further studies are needed to investigate the pathogenic potential and etiological role of these viruses in cattle.

AB - We identified novel viruses in feces from cattle with diarrhea collected in 2009 in Hokkaido Prefecture, Japan, by using a metagenomics approach and determined the (near) complete sequences of the virus. Sequence analyses revealed that they had a standard picornavirus genome organization, i.e. 5' untranslated region (UTR) - L- P1 (VP4- VP3- VP2- VP1) - P2 (2A- 2B- 2C) - P3 (3A- 3B- 3C-3D) - 3'UTR- poly(A). They are closely related to other unclassified Chinese picornaviruses; bat picornaviruses group 1-3, feline picornavirus, and canine picornavirus, sharing 45.4-51.4% (P1), 38.0-44.9% (P2), and 49.6-53.3% (P3) amino acid identities, respectively. The phylogenetic analyses and detailed genome characterization showed that they, together with the unclassified Chinese picornaviruses, grouped as a cluster for the P1, 2C, 3CD and VP1 coding regions. These viruses had conserved features (e.g. predicted protein cleavage sites, presence of a leader protein, 2A, 2C, 3C, and 3D functional domains), suggesting they have a common ancestor. Reverse-transcription-PCR assays, using specific primers designed from the 5'UTR sequence of these viruses, showed that 23.0% (20/87) of fecal samples from cattle with diarrhea were positive, indicating the prevalence of these picornavirus in the Japanese cattle population in Hokkaido Prefecture. However, further studies are needed to investigate the pathogenic potential and etiological role of these viruses in cattle.

KW - Animals

KW - Cattle

KW - Cattle Diseases/epidemiology

KW - Cluster Analysis

KW - Diarrhea/epidemiology

KW - Feces/virology

KW - Gene Order

KW - Genes, Viral

KW - Genome, Viral

KW - Japan/epidemiology

KW - Molecular Sequence Data

KW - Phylogeny

KW - Picornaviridae/classification

KW - Picornaviridae Infections/veterinary

KW - Prevalence

KW - RNA, Viral/genetics

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Analysis, DNA

KW - Sequence Homology

U2 - 10.1016/j.virusres.2015.08.001

DO - 10.1016/j.virusres.2015.08.001

M3 - Journal article

C2 - 26260333

VL - 210

SP - 205

EP - 212

JO - Virus Research

JF - Virus Research

SN - 0168-1702

ER -

ID: 257915576