Horsing around: Escherichia coli ST1250 of equine origin harboring epidemic IncHI1/ST9 plasmid with blaCTX-M-1and an operon for short-chain fructooligosaccharide metabolism
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Horsing around : Escherichia coli ST1250 of equine origin harboring epidemic IncHI1/ST9 plasmid with blaCTX-M-1and an operon for short-chain fructooligosaccharide metabolism. / Valcek, Adam; Sismova, Petra; Nesporova, Kristina; Overballe-Petersen, Søren; Bitar, Ibrahim; Jamborova, Ivana; Kant, Arie; Hrabak, Jaroslav; Wagenaar, Jaap A.; Madec, Jean Yves; Damborg, Peter; Van Duijkeren, Engeline; Ewers, Christa; Hordijk, Joost; Hasman, Henrik; Brouwer, Michael S.M.; Dolejska, Monika.
In: Antimicrobial Agents and Chemotherapy, Vol. 65, No. 5, e02556-20, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Horsing around
T2 - Escherichia coli ST1250 of equine origin harboring epidemic IncHI1/ST9 plasmid with blaCTX-M-1and an operon for short-chain fructooligosaccharide metabolism
AU - Valcek, Adam
AU - Sismova, Petra
AU - Nesporova, Kristina
AU - Overballe-Petersen, Søren
AU - Bitar, Ibrahim
AU - Jamborova, Ivana
AU - Kant, Arie
AU - Hrabak, Jaroslav
AU - Wagenaar, Jaap A.
AU - Madec, Jean Yves
AU - Damborg, Peter
AU - Van Duijkeren, Engeline
AU - Ewers, Christa
AU - Hordijk, Joost
AU - Hasman, Henrik
AU - Brouwer, Michael S.M.
AU - Dolejska, Monika
N1 - Publisher Copyright: © 2021 American Society for Microbiology. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The relatedness of the equine-associated Escherichia coli strain ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of E. coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe (Czech Republic [n = 23], The Netherlands [n = 18], Germany [n = 9], Denmark [n = 3], and France [n = 1]) from 2008 to 2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of E. coli ST1250 and ST1250- SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure, and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 plasmids and one IncHI1/ST2 plasmid were obtained using long-read sequencing by PacBio or MinION. In the collection of 79 isolates, only 10 were phylogenetically close (,8 single nucleotide polymorphisms [SNP]). The majority of isolates belonged to phylogroup B1 (73/79 [92.4%]) and carried blaCTX-M-1(58/79 [73.4%]). The plasmid content of the isolates was dominated by IncHI1 of ST9 (56/62 [90.3%]) and ST2 (6/62 [9.7%]), while 84.5% (49/58) of the blaCTX-M-1genes were associated with the presence of the IncHI1 replicon of ST9 and 6.9% (4/58) with the IncHI1 replicon of ST2 within the corresponding isolates. The operon for the utilization of short-chain fructooligosaccharides (the fos operon) was present in 55 of 79 (69.6%) isolates, and all of these carried IncHI1/ST9 plasmids. The eight complete IncHI1/ST9 plasmid sequences showed the presence of blaCTX-M-1and the fos operon within the same molecule. Sequences of IncHI1/ST9 plasmids were highly conserved (.98% similarity) regardless of country of origin and differed only in the structure and integration site of the multidrug resistance (MDR) region. E. coli ST1250 and ST1250-SLV/DLV are phylogenetically diverse strains associated with horses. A strong linkage of E. coli ST1250 with the epidemic multidrug resistance plasmid lineage IncHI1/ST9 carrying blaCTX-M-1and the fos operon was identified.
AB - The relatedness of the equine-associated Escherichia coli strain ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of E. coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe (Czech Republic [n = 23], The Netherlands [n = 18], Germany [n = 9], Denmark [n = 3], and France [n = 1]) from 2008 to 2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of E. coli ST1250 and ST1250- SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure, and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 plasmids and one IncHI1/ST2 plasmid were obtained using long-read sequencing by PacBio or MinION. In the collection of 79 isolates, only 10 were phylogenetically close (,8 single nucleotide polymorphisms [SNP]). The majority of isolates belonged to phylogroup B1 (73/79 [92.4%]) and carried blaCTX-M-1(58/79 [73.4%]). The plasmid content of the isolates was dominated by IncHI1 of ST9 (56/62 [90.3%]) and ST2 (6/62 [9.7%]), while 84.5% (49/58) of the blaCTX-M-1genes were associated with the presence of the IncHI1 replicon of ST9 and 6.9% (4/58) with the IncHI1 replicon of ST2 within the corresponding isolates. The operon for the utilization of short-chain fructooligosaccharides (the fos operon) was present in 55 of 79 (69.6%) isolates, and all of these carried IncHI1/ST9 plasmids. The eight complete IncHI1/ST9 plasmid sequences showed the presence of blaCTX-M-1and the fos operon within the same molecule. Sequences of IncHI1/ST9 plasmids were highly conserved (.98% similarity) regardless of country of origin and differed only in the structure and integration site of the multidrug resistance (MDR) region. E. coli ST1250 and ST1250-SLV/DLV are phylogenetically diverse strains associated with horses. A strong linkage of E. coli ST1250 with the epidemic multidrug resistance plasmid lineage IncHI1/ST9 carrying blaCTX-M-1and the fos operon was identified.
KW - Escherichia coli
KW - Horses
KW - IncHI1
KW - Multidrug resistance
KW - Plasmids
KW - ST1250
U2 - 10.1128/AAC.02556-20
DO - 10.1128/AAC.02556-20
M3 - Journal article
C2 - 33619063
AN - SCOPUS:85105050921
VL - 65
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 5
M1 - e02556-20
ER -
ID: 262797901