HLA-G expression in placenta in relation to HLA-G genotype and polymorphisms
Research output: Contribution to journal › Journal article › Research › peer-review
PROBLEM: The expression of the non-classical human leukocyte antigen (HLA) class Ib gene, HLA-G, seems to be important at the feto-maternal interface. The HLA-G molecule is almost monomorphic and expressed in both membrane-bound and soluble isoforms. It has been shown to inhibit natural killer cell -mediated lysis and influence cytokine expression. HLA-G gene polymorphism has been linked to differences in gene expression profile of alternatively spliced HLA-G transcripts and levels of specific HLA-G messenger RNA (mRNA) isoforms. Furthermore, aberrant HLA-G expression has been reported in preeclamptic placentas. On this background it is of general interest to further elucidate any associations between HLA-G polymorphism and protein expression.
METHODS: We have investigated HLA-G protein expression by immunohistochemistry in HLA-G genotyped placentas from term. HLA-G mRNA expression in preeclamptic placentas and in control placentas was also studied by microarray technology.
RESULTS AND CONCLUSIONS: The studies of HLA-G protein expression in term placentas by immunohistochemical analysis showed no clear associations with HLA-G genotypes although this could be because of the very semi-quantitative nature of this technique. However, we found a tendency towards reduction of HLA-G mRNA expression in placentas from preeclamptic cases compared to matched controls with the use of microarray technology.
Original language | English |
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Journal | American Journal of Reproductive Immunology Online |
Volume | 52 |
Issue number | 3 |
Pages (from-to) | 212-7 |
Number of pages | 6 |
ISSN | 1046-7408 |
DOIs | |
Publication status | Published - Sep 2004 |
- Biopsy, Female, Genotype, HLA Antigens, HLA-G Antigens, Histocompatibility Antigens Class I, Humans, Immunohistochemistry, Oligonucleotide Array Sequence Analysis, Placenta, Polymorphism, Genetic, Pre-Eclampsia, Pregnancy, Pregnancy Trimester, Third, RNA, Messenger, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 188691711