High reinfection rate and treatment failures in children treated with amodiaquine for falciparum malaria in Muheza villages, Northeastern Tanzania
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High reinfection rate and treatment failures in children treated with amodiaquine for falciparum malaria in Muheza villages, Northeastern Tanzania. / Lemnge, Martha; Alifrangis, Michael; Kafuye, Mwanaidi Y; Segeja, Method D; Gesase, Samwel; Minja, Daniel; Massaga, Julius J; Rønn, Anita M; Bygbjerg, Ib C.
In: American Journal of Tropical Medicine and Hygiene, Vol. 75, No. 2, 2006, p. 188-93.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - High reinfection rate and treatment failures in children treated with amodiaquine for falciparum malaria in Muheza villages, Northeastern Tanzania
AU - Lemnge, Martha
AU - Alifrangis, Michael
AU - Kafuye, Mwanaidi Y
AU - Segeja, Method D
AU - Gesase, Samwel
AU - Minja, Daniel
AU - Massaga, Julius J
AU - Rønn, Anita M
AU - Bygbjerg, Ib C
N1 - Keywords: Amodiaquine; Animals; Antimalarials; Child, Preschool; Cohort Studies; Haplotypes; Hematocrit; Humans; Infant; Malaria, Falciparum; Membrane Proteins; Membrane Transport Proteins; Plasmodium falciparum; Polymerase Chain Reaction; Prevalence; Protozoan Proteins; Recurrence; Tanzania; Treatment Failure
PY - 2006
Y1 - 2006
N2 - In May 2003, we studied amodiaquine (AQ) efficacy in children < 5 years of age with uncomplicated falciparum malaria in Magoda and Mpapayu (with insecticide treated nets [ITNs]) and Mgome (without ITNs) in Muheza, Tanzania. The trial involved 28 days of follow-up, and data were adjusted by polymerase chain reaction (PCR) genotyping of msp1 and msp2 genes. Additionally, Pfcrt codon 72-76 polymorphisms were studied by PCR and sequence-specific oligonucleotide probe (SSOP) ELISA. In 54 cases with complete follow-up, a significant difference in late treatment failure (LTF) rates was seen (60.7% in ITN versus 88.5% in non-ITN villages, P = 0.02) before PCR correction. However, after PCR correction, 23 cases (60.5%) were confirmed as reinfections, giving a true LTF rate of 21.4% (6/28) and 34.6% (9/26) in the above settings, respectively. Frequency of Pfcrt CVIET haplotype mutation pretreatment was high (97.0%); the remaining samples were CVMNK. We conclude that AQ alone is no longer effective in the study area.
AB - In May 2003, we studied amodiaquine (AQ) efficacy in children < 5 years of age with uncomplicated falciparum malaria in Magoda and Mpapayu (with insecticide treated nets [ITNs]) and Mgome (without ITNs) in Muheza, Tanzania. The trial involved 28 days of follow-up, and data were adjusted by polymerase chain reaction (PCR) genotyping of msp1 and msp2 genes. Additionally, Pfcrt codon 72-76 polymorphisms were studied by PCR and sequence-specific oligonucleotide probe (SSOP) ELISA. In 54 cases with complete follow-up, a significant difference in late treatment failure (LTF) rates was seen (60.7% in ITN versus 88.5% in non-ITN villages, P = 0.02) before PCR correction. However, after PCR correction, 23 cases (60.5%) were confirmed as reinfections, giving a true LTF rate of 21.4% (6/28) and 34.6% (9/26) in the above settings, respectively. Frequency of Pfcrt CVIET haplotype mutation pretreatment was high (97.0%); the remaining samples were CVMNK. We conclude that AQ alone is no longer effective in the study area.
M3 - Journal article
C2 - 16896117
VL - 75
SP - 188
EP - 193
JO - Journal. National Malaria Society
JF - Journal. National Malaria Society
SN - 0002-9637
IS - 2
ER -
ID: 9829893