H19 RNA binds four molecules of insulin-like growth factor II mRNA-binding protein
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H19 RNA binds four molecules of insulin-like growth factor II mRNA-binding protein. / Runge, Steffen; Nielsen, Finn Cilius; Nielsen, Jacob; Andersen, Jens Lykke; Wewer, Ulla M.; Christiansen, Jan.
In: Journal of Biological Chemistry, Vol. 275, No. 38, 2000, p. 29562-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - H19 RNA binds four molecules of insulin-like growth factor II mRNA-binding protein
AU - Runge, Steffen
AU - Nielsen, Finn Cilius
AU - Nielsen, Jacob
AU - Andersen, Jens Lykke
AU - Wewer, Ulla M.
AU - Christiansen, Jan
PY - 2000
Y1 - 2000
N2 - H19 RNA is a major oncofetal 2.5-kilobase untranslated RNA of unknown function. The maternally expressed H19 gene is located 90 kilobase pairs downstream from the paternally expressed insulin-like growth factor II (IGF-II) gene on human chromosome 11 and mouse chromosome 7; and due to their reciprocal imprinting and identical spatiotemporal expression, it is assumed that the two genes are functionally coupled. Here we show that human H19 RNA contains four attachment sites for the oncofetal IGF-II mRNA-binding protein (IMP) with apparent K(d) values in the 0.4-1.3 nm range. The multiple attachment sites are clustered within a 700-nucleotide segment encoded by exons 4 and 5. This 3'-terminal segment targets H19 RNA to lamellipodia and perinuclear regions in dispersed fibroblasts where IMP is also localized. The results suggest that IMP participates in H19 RNA localization and provides a link between the IGF-II and H19 genes at post-transcriptional events during mammalian development.
AB - H19 RNA is a major oncofetal 2.5-kilobase untranslated RNA of unknown function. The maternally expressed H19 gene is located 90 kilobase pairs downstream from the paternally expressed insulin-like growth factor II (IGF-II) gene on human chromosome 11 and mouse chromosome 7; and due to their reciprocal imprinting and identical spatiotemporal expression, it is assumed that the two genes are functionally coupled. Here we show that human H19 RNA contains four attachment sites for the oncofetal IGF-II mRNA-binding protein (IMP) with apparent K(d) values in the 0.4-1.3 nm range. The multiple attachment sites are clustered within a 700-nucleotide segment encoded by exons 4 and 5. This 3'-terminal segment targets H19 RNA to lamellipodia and perinuclear regions in dispersed fibroblasts where IMP is also localized. The results suggest that IMP participates in H19 RNA localization and provides a link between the IGF-II and H19 genes at post-transcriptional events during mammalian development.
KW - Animals
KW - Base Sequence
KW - Binding Sites
KW - Humans
KW - Insulin-Like Growth Factor II
KW - Mice
KW - Molecular Sequence Data
KW - Protein Binding
KW - RNA, Messenger
KW - RNA, Untranslated
KW - RNA-Binding Proteins
U2 - 10.1074/jbc.M001156200
DO - 10.1074/jbc.M001156200
M3 - Journal article
C2 - 10875929
VL - 275
SP - 29562
EP - 29569
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 38
ER -
ID: 180674