TY - JOUR

T1 - Glyphosate accumulation, translocation, and biological effects in Coffea arabica after single and multiple exposures

AU - Schrübbers, Lars Christoph

AU - Valverde, Bernal E.

AU - Strobel, Bjarne W.

AU - Cedergreen, Nina

PY - 2016

Y1 - 2016

N2 - In perennial crops like coffee, glyphosate drift exposure can occur multiple times during its commercial life span. Due to limited glyphosate degradation in higher plants, a potential accumulation of glyphosate could lead to increased biological effects with increased exposure frequency. In this study, we investigated glyphosate translocation over time, and its concentration and biological effects after single and multiple simulated spray-drift exposures. Additionally, shikimic acid/glyphosate ratios were used as biomarkers for glyphosate binding to its target enzyme.Four weeks after the exposure, glyphosate was continuously translocated. Shikimic acid levels were lin-ear correlated with glyphosate levels. After two months, however, glyphosate appeared to have reduced activity. In the greenhouse, multiple applications resulted in higher internal glyphosate concentrations.The time of application, however, was more important regarding biological effects than the number of applications both in the greenhouse and in the field. In the field, berry yield, the most important biological response variable, was reduced 26% by the first out of four sequential applications of glyphosate at 64 g a.e. ha− 1each. The three subsequent applications did not reduce yield any further.

AB - In perennial crops like coffee, glyphosate drift exposure can occur multiple times during its commercial life span. Due to limited glyphosate degradation in higher plants, a potential accumulation of glyphosate could lead to increased biological effects with increased exposure frequency. In this study, we investigated glyphosate translocation over time, and its concentration and biological effects after single and multiple simulated spray-drift exposures. Additionally, shikimic acid/glyphosate ratios were used as biomarkers for glyphosate binding to its target enzyme.Four weeks after the exposure, glyphosate was continuously translocated. Shikimic acid levels were lin-ear correlated with glyphosate levels. After two months, however, glyphosate appeared to have reduced activity. In the greenhouse, multiple applications resulted in higher internal glyphosate concentrations.The time of application, however, was more important regarding biological effects than the number of applications both in the greenhouse and in the field. In the field, berry yield, the most important biological response variable, was reduced 26% by the first out of four sequential applications of glyphosate at 64 g a.e. ha− 1each. The three subsequent applications did not reduce yield any further.

U2 - 10.1016/j.eja.2015.11.023

DO - 10.1016/j.eja.2015.11.023

M3 - Journal article

VL - 74

SP - 133

EP - 143

JO - European Journal of Agronomy

JF - European Journal of Agronomy

SN - 1161-0301

ER -