GluR2 ligand-binding core complexes: importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

GluR2 ligand-binding core complexes : importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists. / Kasper, C; Lunn, M-L; Liljefors, T; Gouaux, E; Egebjerg, J; Kastrup, Jette Sandholm Jensen.

In: F E B S Letters, Vol. 531, No. 2, 06.11.2002, p. 173-8.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kasper, C, Lunn, M-L, Liljefors, T, Gouaux, E, Egebjerg, J & Kastrup, JSJ 2002, 'GluR2 ligand-binding core complexes: importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists', F E B S Letters, vol. 531, no. 2, pp. 173-8.

APA

Kasper, C., Lunn, M-L., Liljefors, T., Gouaux, E., Egebjerg, J., & Kastrup, J. S. J. (2002). GluR2 ligand-binding core complexes: importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists. F E B S Letters, 531(2), 173-8.

Vancouver

Kasper C, Lunn M-L, Liljefors T, Gouaux E, Egebjerg J, Kastrup JSJ. GluR2 ligand-binding core complexes: importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists. F E B S Letters. 2002 Nov 6;531(2):173-8.

Author

Kasper, C ; Lunn, M-L ; Liljefors, T ; Gouaux, E ; Egebjerg, J ; Kastrup, Jette Sandholm Jensen. / GluR2 ligand-binding core complexes : importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists. In: F E B S Letters. 2002 ; Vol. 531, No. 2. pp. 173-8.

Bibtex

@article{2401925dcd064c1f8a0fb05ba5c3dca6,
title = "GluR2 ligand-binding core complexes: importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists",
abstract = "X-ray structures of the GluR2 ligand-binding core in complex with (S)-Des-Me-AMPA and in the presence and absence of zinc ions have been determined. (S)-Des-Me-AMPA, which is devoid of a substituent in the 5-position of the isoxazolol ring, only has limited interactions with the partly hydrophobic pocket of the ligand-binding site, and adopts an AMPA-like binding mode. The structures, in comparison with other agonist complex structures, disclose the relative importance of the isoxazolol ring and of the substituent in the 5-position for the mode of binding. A relationship appears to exist between the extent of interaction of the ligand with the hydrophobic pocket and the affinity of the ligand.",
keywords = "Animals, Binding Sites, Crystallography, X-Ray, Hydrogen Bonding, Hydrophobic and Hydrophilic Interactions, Isoxazoles, Ligands, Macromolecular Substances, Methionine, Models, Molecular, Peptides, Protein Binding, Receptors, AMPA, Sulfates, Zinc, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid",
author = "C Kasper and M-L Lunn and T Liljefors and E Gouaux and J Egebjerg and Kastrup, {Jette Sandholm Jensen}",
year = "2002",
month = "11",
day = "6",
language = "English",
volume = "531",
pages = "173--8",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - GluR2 ligand-binding core complexes

T2 - importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists

AU - Kasper, C

AU - Lunn, M-L

AU - Liljefors, T

AU - Gouaux, E

AU - Egebjerg, J

AU - Kastrup, Jette Sandholm Jensen

PY - 2002/11/6

Y1 - 2002/11/6

N2 - X-ray structures of the GluR2 ligand-binding core in complex with (S)-Des-Me-AMPA and in the presence and absence of zinc ions have been determined. (S)-Des-Me-AMPA, which is devoid of a substituent in the 5-position of the isoxazolol ring, only has limited interactions with the partly hydrophobic pocket of the ligand-binding site, and adopts an AMPA-like binding mode. The structures, in comparison with other agonist complex structures, disclose the relative importance of the isoxazolol ring and of the substituent in the 5-position for the mode of binding. A relationship appears to exist between the extent of interaction of the ligand with the hydrophobic pocket and the affinity of the ligand.

AB - X-ray structures of the GluR2 ligand-binding core in complex with (S)-Des-Me-AMPA and in the presence and absence of zinc ions have been determined. (S)-Des-Me-AMPA, which is devoid of a substituent in the 5-position of the isoxazolol ring, only has limited interactions with the partly hydrophobic pocket of the ligand-binding site, and adopts an AMPA-like binding mode. The structures, in comparison with other agonist complex structures, disclose the relative importance of the isoxazolol ring and of the substituent in the 5-position for the mode of binding. A relationship appears to exist between the extent of interaction of the ligand with the hydrophobic pocket and the affinity of the ligand.

KW - Animals

KW - Binding Sites

KW - Crystallography, X-Ray

KW - Hydrogen Bonding

KW - Hydrophobic and Hydrophilic Interactions

KW - Isoxazoles

KW - Ligands

KW - Macromolecular Substances

KW - Methionine

KW - Models, Molecular

KW - Peptides

KW - Protein Binding

KW - Receptors, AMPA

KW - Sulfates

KW - Zinc

KW - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid

M3 - Journal article

C2 - 12417307

VL - 531

SP - 173

EP - 178

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 2

ER -

ID: 44729582