Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
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Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections. / García, Vanesa; Grønnemose, Rasmus B.; Torres-Puig, Sergi; Kudirkiene, Egle; Piantelli, Mateo; Ahmed, Shahana; Andersen, Thomas E.; Møller-Jensen, Jakob; Olsen, John E.; Herrero-Fresno, Ana.
In: Microbial Genomics, Vol. 7, No. 12, :000719, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections
AU - García, Vanesa
AU - Grønnemose, Rasmus B.
AU - Torres-Puig, Sergi
AU - Kudirkiene, Egle
AU - Piantelli, Mateo
AU - Ahmed, Shahana
AU - Andersen, Thomas E.
AU - Møller-Jensen, Jakob
AU - Olsen, John E.
AU - Herrero-Fresno, Ana
PY - 2021
Y1 - 2021
N2 - Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approach (TraDIS) to reveal the UTI89 essential-genes for in vitro growth and fitness-gene-sets for growth in Luria broth (LB) and EZ-MOPS medium without glucose, as well as for human bacteriuria and mouse cystitis. A total of 293 essential genes for growth were identified and the set of fitness-genes was shown to differ depending on the growth media. A modified, previously validated UTI murine model, with administration of glucose prior to infection was applied. Selected fitness-genes for growth in urine and mouse-bladder colonization were validated using deletion-mutants. Novel fitness-genes, such as tusA, corA and rfaG; involved in sulphur-acquisition, magnesium-uptake, and LPS-biosynthesis, were proved to be important during UTI. Moreover, rfaG was confirmed as relevant in both niches, and therefore it may represent a target for novel UTI-treatment/prevention strategies.
AB - Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approach (TraDIS) to reveal the UTI89 essential-genes for in vitro growth and fitness-gene-sets for growth in Luria broth (LB) and EZ-MOPS medium without glucose, as well as for human bacteriuria and mouse cystitis. A total of 293 essential genes for growth were identified and the set of fitness-genes was shown to differ depending on the growth media. A modified, previously validated UTI murine model, with administration of glucose prior to infection was applied. Selected fitness-genes for growth in urine and mouse-bladder colonization were validated using deletion-mutants. Novel fitness-genes, such as tusA, corA and rfaG; involved in sulphur-acquisition, magnesium-uptake, and LPS-biosynthesis, were proved to be important during UTI. Moreover, rfaG was confirmed as relevant in both niches, and therefore it may represent a target for novel UTI-treatment/prevention strategies.
KW - essentiality
KW - EZ-MOPS
KW - fitness
KW - human bacteriuria
KW - LB
KW - mouse cystitis
KW - TraDIS
KW - urinary tract infection
KW - Uropathogenic E. coli
U2 - 10.1099/mgen.0.000719
DO - 10.1099/mgen.0.000719
M3 - Journal article
C2 - 34928200
AN - SCOPUS:85122904039
VL - 7
JO - Microbial Genomics
JF - Microbial Genomics
SN - 2057-5858
IS - 12
M1 - :000719
ER -
ID: 299205886