Functional properties of human neuronal Kv11 channels
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Functional properties of human neuronal Kv11 channels. / Einarsen, Karoline; Calloe, Kirstine; Grunnet, Morten; Olesen, Søren-Peter; Schmitt, Nicole.
In: Pflügers Archiv: European Journal of Physiology, Vol. 458, No. 4, 2009, p. 689-700.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Functional properties of human neuronal Kv11 channels
AU - Einarsen, Karoline
AU - Calloe, Kirstine
AU - Grunnet, Morten
AU - Olesen, Søren-Peter
AU - Schmitt, Nicole
N1 - Keywords: Action Potentials; Animals; Cells, Cultured; Ion Channel Gating; Membrane Potentials; Neurons; Oocytes; Potassium Channels; Xenopus laevis
PY - 2009
Y1 - 2009
N2 - Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present a new splice variant that is present in human brain as demonstrated by reverse transcription PCR. Heterologous expression in Xenopus laevis oocytes revealed a 30-mV shift in the voltage dependence of activation to more depolarized potentials and slower activation together with faster deactivation kinetics compared to hKv11.1. Further, we have cloned and electrophysiologically characterized two splice variants of hKv11.3. When expressed in X. laevis oocytes, both isoform 1 and isoform 2 elicited robust currents with a striking transient current component caused by delayed inactivation. The different current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability.
AB - Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present a new splice variant that is present in human brain as demonstrated by reverse transcription PCR. Heterologous expression in Xenopus laevis oocytes revealed a 30-mV shift in the voltage dependence of activation to more depolarized potentials and slower activation together with faster deactivation kinetics compared to hKv11.1. Further, we have cloned and electrophysiologically characterized two splice variants of hKv11.3. When expressed in X. laevis oocytes, both isoform 1 and isoform 2 elicited robust currents with a striking transient current component caused by delayed inactivation. The different current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability.
U2 - 10.1007/s00424-009-0651-5
DO - 10.1007/s00424-009-0651-5
M3 - Journal article
C2 - 19319565
VL - 458
SP - 689
EP - 700
JO - Pflügers Archiv - European Journal of Physiology
JF - Pflügers Archiv - European Journal of Physiology
SN - 0031-6768
IS - 4
ER -
ID: 18699137