Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle. / Frees, Dorte; Ingmer, Hanne.

In: mBio, Vol. 13, No. 4, e0073722, 2022.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Frees, D & Ingmer, H 2022, 'Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle', mBio, vol. 13, no. 4, e0073722. https://doi.org/10.1128/mbio.00737-22

APA

Frees, D., & Ingmer, H. (2022). Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle. mBio, 13(4), [e0073722]. https://doi.org/10.1128/mbio.00737-22

Vancouver

Frees D, Ingmer H. Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle. mBio. 2022;13(4). e0073722. https://doi.org/10.1128/mbio.00737-22

Author

Frees, Dorte ; Ingmer, Hanne. / Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle. In: mBio. 2022 ; Vol. 13, No. 4.

Bibtex

@article{3a780073ba2c482880d4263a5494b0fa,
title = "Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle",
abstract = "The division of bacterial cells into two daughter cells requires a precise balance of more than a dozen highly conserved proteins that coordinate chromosome segregation with the synthesis of the novel cell envelope. The paradigms of cell division were established in rod-shaped bacteria and this fundamental process is far less characterized in spherical bacteria. In a search for novel, essential cell division proteins in Staphylococci, Myrbr{\aa}ten et al. used combined depletion and subcellular localization analyses to identify the staphylococcal morphology determinant, SmdA, that is exclusively found in cocci. Knockdown of smdA results in severe division defects and increased sensitivity to cell wall targeting antibiotics. Although determining the precise role of SmdA in S. aureus cell division will require further research, this study provides a striking example of how researchers can assign functions to genes that are too fundamental to cell biology to allow genetic inactivation.",
keywords = "cell division, CRISPRi, essential genes, MRSA, PBP1, Staphylococcus aureus",
author = "Dorte Frees and Hanne Ingmer",
year = "2022",
doi = "10.1128/mbio.00737-22",
language = "English",
volume = "13",
journal = "mBio",
issn = "2161-2129",
publisher = "American Society for Microbiology",
number = "4",

}

RIS

TY - JOUR

T1 - Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle

AU - Frees, Dorte

AU - Ingmer, Hanne

PY - 2022

Y1 - 2022

N2 - The division of bacterial cells into two daughter cells requires a precise balance of more than a dozen highly conserved proteins that coordinate chromosome segregation with the synthesis of the novel cell envelope. The paradigms of cell division were established in rod-shaped bacteria and this fundamental process is far less characterized in spherical bacteria. In a search for novel, essential cell division proteins in Staphylococci, Myrbråten et al. used combined depletion and subcellular localization analyses to identify the staphylococcal morphology determinant, SmdA, that is exclusively found in cocci. Knockdown of smdA results in severe division defects and increased sensitivity to cell wall targeting antibiotics. Although determining the precise role of SmdA in S. aureus cell division will require further research, this study provides a striking example of how researchers can assign functions to genes that are too fundamental to cell biology to allow genetic inactivation.

AB - The division of bacterial cells into two daughter cells requires a precise balance of more than a dozen highly conserved proteins that coordinate chromosome segregation with the synthesis of the novel cell envelope. The paradigms of cell division were established in rod-shaped bacteria and this fundamental process is far less characterized in spherical bacteria. In a search for novel, essential cell division proteins in Staphylococci, Myrbråten et al. used combined depletion and subcellular localization analyses to identify the staphylococcal morphology determinant, SmdA, that is exclusively found in cocci. Knockdown of smdA results in severe division defects and increased sensitivity to cell wall targeting antibiotics. Although determining the precise role of SmdA in S. aureus cell division will require further research, this study provides a striking example of how researchers can assign functions to genes that are too fundamental to cell biology to allow genetic inactivation.

KW - cell division

KW - CRISPRi

KW - essential genes

KW - MRSA

KW - PBP1

KW - Staphylococcus aureus

U2 - 10.1128/mbio.00737-22

DO - 10.1128/mbio.00737-22

M3 - Journal article

C2 - 35735744

AN - SCOPUS:85137127258

VL - 13

JO - mBio

JF - mBio

SN - 2161-2129

IS - 4

M1 - e0073722

ER -

ID: 319399114