Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle. / Frees, Dorte; Ingmer, Hanne.
In: mBio, Vol. 13, No. 4, e0073722, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Finding New Fundamental Pieces for the Bacterial Cell Division Puzzle
AU - Frees, Dorte
AU - Ingmer, Hanne
PY - 2022
Y1 - 2022
N2 - The division of bacterial cells into two daughter cells requires a precise balance of more than a dozen highly conserved proteins that coordinate chromosome segregation with the synthesis of the novel cell envelope. The paradigms of cell division were established in rod-shaped bacteria and this fundamental process is far less characterized in spherical bacteria. In a search for novel, essential cell division proteins in Staphylococci, Myrbråten et al. used combined depletion and subcellular localization analyses to identify the staphylococcal morphology determinant, SmdA, that is exclusively found in cocci. Knockdown of smdA results in severe division defects and increased sensitivity to cell wall targeting antibiotics. Although determining the precise role of SmdA in S. aureus cell division will require further research, this study provides a striking example of how researchers can assign functions to genes that are too fundamental to cell biology to allow genetic inactivation.
AB - The division of bacterial cells into two daughter cells requires a precise balance of more than a dozen highly conserved proteins that coordinate chromosome segregation with the synthesis of the novel cell envelope. The paradigms of cell division were established in rod-shaped bacteria and this fundamental process is far less characterized in spherical bacteria. In a search for novel, essential cell division proteins in Staphylococci, Myrbråten et al. used combined depletion and subcellular localization analyses to identify the staphylococcal morphology determinant, SmdA, that is exclusively found in cocci. Knockdown of smdA results in severe division defects and increased sensitivity to cell wall targeting antibiotics. Although determining the precise role of SmdA in S. aureus cell division will require further research, this study provides a striking example of how researchers can assign functions to genes that are too fundamental to cell biology to allow genetic inactivation.
KW - cell division
KW - CRISPRi
KW - essential genes
KW - MRSA
KW - PBP1
KW - Staphylococcus aureus
U2 - 10.1128/mbio.00737-22
DO - 10.1128/mbio.00737-22
M3 - Journal article
C2 - 35735744
AN - SCOPUS:85137127258
VL - 13
JO - mBio
JF - mBio
SN - 2161-2129
IS - 4
M1 - e0073722
ER -
ID: 319399114