TY - JOUR

T1 - Few X-ray and PUVA treatments accelerate photocarcinogenesis in hairless mice

AU - Lerche, Catharina M.

AU - Al-Chaer, Rami Nabil

AU - Glud, Martin

AU - Philipsen, Peter Alshede

AU - Wulf, Hans Christian

N1 - Funding Information: The research was supported by Copenhagen University Hospital, Bispebjerg and Kgl. Hofbundtmager Aage Bangs Foundation. C.M.L is supported by an unrestricted grant from the Lundbeck Foundation (R307-2018-3318). Funding Information: The authors extend their gratitude to the nurses from the Department of Dermatology D41 for helping with the X-ray treatments and M.Sc. E.E. Jakob Heydenreich for recording the spectrum of the UVA source.?This work was executed as a part of the Danish Research Center for Skin Cancer and the Skin Cancer Innovation Clinical Academic Group (SCIN CAG), Greater Copenhagen Health Science Partners (GCHSP). Publisher Copyright: © 2021, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.

PY - 2021

Y1 - 2021

N2 - PUVA is a treatment that combines oral methoxypsoralen (8-MOP) with ultraviolet radiation A (UVA). It is used for severe psoriasis and the early stages of T-cell lymphoma. X-rays are an effective treatment for skin cancers. Both treatments are in higher doses used to treat skin malignancies and simultaneously increase the risk of keratinocyte cancer. The main objective of this study was to test whether a few PUVA or X-ray treatments could delay the development of ultraviolet radiation (UVR)-induced skin tumors in a well-established hairless mouse model. Three groups of immunocompetent mice (total, N = 75) were included in the study. All groups were UVR-exposed during the study period. In addition, one group was treated with PUVA and another group was treated with X-rays at days 45, 52, 90 and 97. A control group was treated with UVR only. We recorded when the first, second and third skin tumors were induced in each mouse. Skin tumors developed significantly earlier in both the PUVA and X-ray groups (median, 188 days) than in the control mice (median, 215 days; p < 0.001). Therefore, a few X-ray and PUVA treatments both significantly accelerated the development of skin tumors in hairless mice, compared to UVR controls. Neither treatment showed a delay of UVR-induced skin tumors and caution should be exercised before applying these treatments to sun-damaged skin. Graphic abstract: [Figure not available: see fulltext.].

AB - PUVA is a treatment that combines oral methoxypsoralen (8-MOP) with ultraviolet radiation A (UVA). It is used for severe psoriasis and the early stages of T-cell lymphoma. X-rays are an effective treatment for skin cancers. Both treatments are in higher doses used to treat skin malignancies and simultaneously increase the risk of keratinocyte cancer. The main objective of this study was to test whether a few PUVA or X-ray treatments could delay the development of ultraviolet radiation (UVR)-induced skin tumors in a well-established hairless mouse model. Three groups of immunocompetent mice (total, N = 75) were included in the study. All groups were UVR-exposed during the study period. In addition, one group was treated with PUVA and another group was treated with X-rays at days 45, 52, 90 and 97. A control group was treated with UVR only. We recorded when the first, second and third skin tumors were induced in each mouse. Skin tumors developed significantly earlier in both the PUVA and X-ray groups (median, 188 days) than in the control mice (median, 215 days; p < 0.001). Therefore, a few X-ray and PUVA treatments both significantly accelerated the development of skin tumors in hairless mice, compared to UVR controls. Neither treatment showed a delay of UVR-induced skin tumors and caution should be exercised before applying these treatments to sun-damaged skin. Graphic abstract: [Figure not available: see fulltext.].

KW - 8-MOP

KW - Hairless mice

KW - Prophylactic treatment

KW - PUVA

KW - Radiation therapy

KW - Skin tumors

KW - Ultraviolet radiation

KW - X-ray

U2 - 10.1007/s43630-021-00105-y

DO - 10.1007/s43630-021-00105-y

M3 - Journal article

C2 - 34559384

AN - SCOPUS:85115655401

VL - 20

SP - 1299

EP - 1307

JO - Photochemical & Photobiological Sciences

JF - Photochemical & Photobiological Sciences

SN - 1474-905X

IS - 10

ER -