TY - JOUR

T1 - Extraction performance of electromembrane extraction and liquid-phase microextraction in prototype equipment

AU - Schüller, Maria

AU - Hansen, Frederik André

AU - Pedersen-Bjergaard, Stig

N1 - Publisher Copyright: © 2023 The Author(s)

PY - 2023

Y1 - 2023

N2 - In this comparative study, the performance of liquid-phase microextraction and electromembrane extraction in prototype equipment was evaluated for extraction of ninety basic substances from plasma. Using a commercial EME device based on conductive vials enabled a standardized and comprehensive comparison between the two methods. Extractions were performed from a pH-adjusted donor solution, across an organic liquid membrane immobilized in a porous polypropylene membrane, and into an acidic acceptor solution. In LPME, dodecyl acetate was used as the extraction solvent, while 2-nitrophenyl octyl ether was used for EME with an electric field applied across the system. To assess the extraction performance, extraction recovery plots and extraction time curves were constructed and analyzed. These plots provided insights into the efficiency and effectiveness of LPME and EME, allowing users to make better decisions about the most suitable method for a specific bioanalytical application. Both LPME and EME were effective for substances with 2.0 < log P < 4.0, with EME showing faster extraction kinetics. Small (200 µL) and large vials (600 µL) were compared, showing that smaller vials improved kinetics markedly in both techniques. Carrier-mediated extraction showed improved performance for analytes with log P < 2 in EME, however, with some limitations due to system instability. This is, to our knowledge, the first time LPME was performed in the commercial vial-based equipment. An evaluation of vial-based LPME investigating linearity, precision, accuracy, and matrix effects showed promising results. These findings contribute to a general understanding of the performance differences in vial-based LPME and EME.

AB - In this comparative study, the performance of liquid-phase microextraction and electromembrane extraction in prototype equipment was evaluated for extraction of ninety basic substances from plasma. Using a commercial EME device based on conductive vials enabled a standardized and comprehensive comparison between the two methods. Extractions were performed from a pH-adjusted donor solution, across an organic liquid membrane immobilized in a porous polypropylene membrane, and into an acidic acceptor solution. In LPME, dodecyl acetate was used as the extraction solvent, while 2-nitrophenyl octyl ether was used for EME with an electric field applied across the system. To assess the extraction performance, extraction recovery plots and extraction time curves were constructed and analyzed. These plots provided insights into the efficiency and effectiveness of LPME and EME, allowing users to make better decisions about the most suitable method for a specific bioanalytical application. Both LPME and EME were effective for substances with 2.0 < log P < 4.0, with EME showing faster extraction kinetics. Small (200 µL) and large vials (600 µL) were compared, showing that smaller vials improved kinetics markedly in both techniques. Carrier-mediated extraction showed improved performance for analytes with log P < 2 in EME, however, with some limitations due to system instability. This is, to our knowledge, the first time LPME was performed in the commercial vial-based equipment. An evaluation of vial-based LPME investigating linearity, precision, accuracy, and matrix effects showed promising results. These findings contribute to a general understanding of the performance differences in vial-based LPME and EME.

KW - Carrier-mediated extraction

KW - Electromembrane extraction

KW - Liquid-phase microextraction

KW - Pharmaceuticals

KW - Plasma samples

KW - Time curves

U2 - 10.1016/j.chroma.2023.464440

DO - 10.1016/j.chroma.2023.464440

M3 - Journal article

C2 - 37832461

AN - SCOPUS:85173297088

VL - 1710

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0301-4770

M1 - 464440

ER -