Extraction and purification of viruses from fecal samples for metagenome and morphology analyses

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Extraction and purification of viruses from fecal samples for metagenome and morphology analyses. / Castro-Mejía, Josué L.; Deng, Ling; Vogensen, Finn K.; Reyes, Alejandro; Nielsen, Dennis S.

The Human Virome: Methods and Protocols. ed. / Andrés Moya; Vicente Pérez Brocal. Humana Press, 2018. p. 49-57 (Methods in Molecular Biology, Vol. 1838).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Castro-Mejía, JL, Deng, L, Vogensen, FK, Reyes, A & Nielsen, DS 2018, Extraction and purification of viruses from fecal samples for metagenome and morphology analyses. in A Moya & VP Brocal (eds), The Human Virome: Methods and Protocols. Humana Press, Methods in Molecular Biology, vol. 1838, pp. 49-57. https://doi.org/10.1007/978-1-4939-8682-8_5

APA

Castro-Mejía, J. L., Deng, L., Vogensen, F. K., Reyes, A., & Nielsen, D. S. (2018). Extraction and purification of viruses from fecal samples for metagenome and morphology analyses. In A. Moya, & V. P. Brocal (Eds.), The Human Virome: Methods and Protocols (pp. 49-57). Humana Press. Methods in Molecular Biology Vol. 1838 https://doi.org/10.1007/978-1-4939-8682-8_5

Vancouver

Castro-Mejía JL, Deng L, Vogensen FK, Reyes A, Nielsen DS. Extraction and purification of viruses from fecal samples for metagenome and morphology analyses. In Moya A, Brocal VP, editors, The Human Virome: Methods and Protocols. Humana Press. 2018. p. 49-57. (Methods in Molecular Biology, Vol. 1838). https://doi.org/10.1007/978-1-4939-8682-8_5

Author

Castro-Mejía, Josué L. ; Deng, Ling ; Vogensen, Finn K. ; Reyes, Alejandro ; Nielsen, Dennis S. / Extraction and purification of viruses from fecal samples for metagenome and morphology analyses. The Human Virome: Methods and Protocols. editor / Andrés Moya ; Vicente Pérez Brocal. Humana Press, 2018. pp. 49-57 (Methods in Molecular Biology, Vol. 1838).

Bibtex

@inbook{b289f8db063341ec8d8feee2b6fe62d1,
title = "Extraction and purification of viruses from fecal samples for metagenome and morphology analyses",
abstract = "The human enteric virome consists of endogenous retro elements and viruses that infect the host and members of the gut microbiome (GM). Mounting evidence suggests that the gut virome plays a central role in maintaining homeostasis and via the GM influences immunology of the host. To thoroughly characterize the gut virome, it is often very useful to first separate and concentrate extracellular viral-like particles (eVLPs) enabling an integrative characterization of them. Here, we describe a detailed protocol for extraction and concentration of the viral fraction from fecal samples based on a polyethylene glycol precipitation (PEG) approach. These procedures maximize the yields of eVLPs (and their DNA) with high purity well suited for down-stream analysis such as quantification and morphological assessment, determination of phage-host pairs as well as virome sequencing.",
keywords = "Extraction procedures, Gut-virome, Metagenome of extracellular viral-like particles (eVLPs), Morphology, Polyethylene-glycol (PEG) precipitation",
author = "Castro-Mej{\'i}a, {Josu{\'e} L.} and Ling Deng and Vogensen, {Finn K.} and Alejandro Reyes and Nielsen, {Dennis S.}",
year = "2018",
doi = "10.1007/978-1-4939-8682-8_5",
language = "English",
isbn = " 978-1-4939-8681-1",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "49--57",
editor = "Andr{\'e}s Moya and Brocal, {Vicente P{\'e}rez}",
booktitle = "The Human Virome",
address = "United States",

}

RIS

TY - CHAP

T1 - Extraction and purification of viruses from fecal samples for metagenome and morphology analyses

AU - Castro-Mejía, Josué L.

AU - Deng, Ling

AU - Vogensen, Finn K.

AU - Reyes, Alejandro

AU - Nielsen, Dennis S.

PY - 2018

Y1 - 2018

N2 - The human enteric virome consists of endogenous retro elements and viruses that infect the host and members of the gut microbiome (GM). Mounting evidence suggests that the gut virome plays a central role in maintaining homeostasis and via the GM influences immunology of the host. To thoroughly characterize the gut virome, it is often very useful to first separate and concentrate extracellular viral-like particles (eVLPs) enabling an integrative characterization of them. Here, we describe a detailed protocol for extraction and concentration of the viral fraction from fecal samples based on a polyethylene glycol precipitation (PEG) approach. These procedures maximize the yields of eVLPs (and their DNA) with high purity well suited for down-stream analysis such as quantification and morphological assessment, determination of phage-host pairs as well as virome sequencing.

AB - The human enteric virome consists of endogenous retro elements and viruses that infect the host and members of the gut microbiome (GM). Mounting evidence suggests that the gut virome plays a central role in maintaining homeostasis and via the GM influences immunology of the host. To thoroughly characterize the gut virome, it is often very useful to first separate and concentrate extracellular viral-like particles (eVLPs) enabling an integrative characterization of them. Here, we describe a detailed protocol for extraction and concentration of the viral fraction from fecal samples based on a polyethylene glycol precipitation (PEG) approach. These procedures maximize the yields of eVLPs (and their DNA) with high purity well suited for down-stream analysis such as quantification and morphological assessment, determination of phage-host pairs as well as virome sequencing.

KW - Extraction procedures

KW - Gut-virome

KW - Metagenome of extracellular viral-like particles (eVLPs)

KW - Morphology

KW - Polyethylene-glycol (PEG) precipitation

U2 - 10.1007/978-1-4939-8682-8_5

DO - 10.1007/978-1-4939-8682-8_5

M3 - Book chapter

C2 - 30128989

AN - SCOPUS:85052194015

SN - 978-1-4939-8681-1

T3 - Methods in Molecular Biology

SP - 49

EP - 57

BT - The Human Virome

A2 - Moya, Andrés

A2 - Brocal, Vicente Pérez

PB - Humana Press

ER -

ID: 210012489