Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells

Research output: Contribution to journalJournal articlepeer-review

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Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. / Theurillat, Ilan; Hendriks, Ivo A.; Cossec, Jack-Christophe; Andrieux, Alexandra; Nielsen, Michael L.; Dejean, Anne.

In: Cell Reports, Vol. 32, No. 11, 108146, 2020.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Theurillat, I, Hendriks, IA, Cossec, J-C, Andrieux, A, Nielsen, ML & Dejean, A 2020, 'Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells', Cell Reports, vol. 32, no. 11, 108146. https://doi.org/10.1016/j.celrep.2020.108146

APA

Theurillat, I., Hendriks, I. A., Cossec, J-C., Andrieux, A., Nielsen, M. L., & Dejean, A. (2020). Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. Cell Reports, 32(11), [108146]. https://doi.org/10.1016/j.celrep.2020.108146

Vancouver

Theurillat I, Hendriks IA, Cossec J-C, Andrieux A, Nielsen ML, Dejean A. Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. Cell Reports. 2020;32(11). 108146. https://doi.org/10.1016/j.celrep.2020.108146

Author

Theurillat, Ilan ; Hendriks, Ivo A. ; Cossec, Jack-Christophe ; Andrieux, Alexandra ; Nielsen, Michael L. ; Dejean, Anne. / Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. In: Cell Reports. 2020 ; Vol. 32, No. 11.

Bibtex

@article{a74ea0bc1b444e8487da80c26425b31d,
title = "Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells",
abstract = "Post-translational modification by SUMO is a key regulator of cell identity. In mouse embryonic fibroblasts (MEFs), SUMO impedes reprogramming to pluripotency, while in embryonic stem cells (ESCs), it represses the emergence of totipotent-like cells, suggesting that SUMO targets distinct substrates to preserve somatic and pluripotent states. Using MS-based proteomics, we show that the composition of endogenous SUMOylomes differs dramatically between MEFs and ESCs. In MEFs, SUMO2/3 targets proteins associated with canonical SUMO functions, such as splicing, and transcriptional regulators driving somatic enhancer selection. In contrast, in ESCs, SUMO2/3 primarily modifies highly interconnected repressive chromatin complexes, thereby preventing chromatin opening and transitioning to totipotent-like states. We also characterize several SUMO-modified pluripotency factors and show that SUMOylation of Dppa2 and Dppa4 impedes the conversion to 2-cell-embryo-like states. Altogether, we propose that rewiring the repertoire of SUMO target networks is a major driver of cell fate decision during embryonic development.",
keywords = "TRANSCRIPTION FACTORS, SUMOYLATION, IDENTIFICATION, INSIGHTS, PATHWAY, COMPLEX, BINDING",
author = "Ilan Theurillat and Hendriks, {Ivo A.} and Jack-Christophe Cossec and Alexandra Andrieux and Nielsen, {Michael L.} and Anne Dejean",
year = "2020",
doi = "10.1016/j.celrep.2020.108146",
language = "English",
volume = "32",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "11",

}

RIS

TY - JOUR

T1 - Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells

AU - Theurillat, Ilan

AU - Hendriks, Ivo A.

AU - Cossec, Jack-Christophe

AU - Andrieux, Alexandra

AU - Nielsen, Michael L.

AU - Dejean, Anne

PY - 2020

Y1 - 2020

N2 - Post-translational modification by SUMO is a key regulator of cell identity. In mouse embryonic fibroblasts (MEFs), SUMO impedes reprogramming to pluripotency, while in embryonic stem cells (ESCs), it represses the emergence of totipotent-like cells, suggesting that SUMO targets distinct substrates to preserve somatic and pluripotent states. Using MS-based proteomics, we show that the composition of endogenous SUMOylomes differs dramatically between MEFs and ESCs. In MEFs, SUMO2/3 targets proteins associated with canonical SUMO functions, such as splicing, and transcriptional regulators driving somatic enhancer selection. In contrast, in ESCs, SUMO2/3 primarily modifies highly interconnected repressive chromatin complexes, thereby preventing chromatin opening and transitioning to totipotent-like states. We also characterize several SUMO-modified pluripotency factors and show that SUMOylation of Dppa2 and Dppa4 impedes the conversion to 2-cell-embryo-like states. Altogether, we propose that rewiring the repertoire of SUMO target networks is a major driver of cell fate decision during embryonic development.

AB - Post-translational modification by SUMO is a key regulator of cell identity. In mouse embryonic fibroblasts (MEFs), SUMO impedes reprogramming to pluripotency, while in embryonic stem cells (ESCs), it represses the emergence of totipotent-like cells, suggesting that SUMO targets distinct substrates to preserve somatic and pluripotent states. Using MS-based proteomics, we show that the composition of endogenous SUMOylomes differs dramatically between MEFs and ESCs. In MEFs, SUMO2/3 targets proteins associated with canonical SUMO functions, such as splicing, and transcriptional regulators driving somatic enhancer selection. In contrast, in ESCs, SUMO2/3 primarily modifies highly interconnected repressive chromatin complexes, thereby preventing chromatin opening and transitioning to totipotent-like states. We also characterize several SUMO-modified pluripotency factors and show that SUMOylation of Dppa2 and Dppa4 impedes the conversion to 2-cell-embryo-like states. Altogether, we propose that rewiring the repertoire of SUMO target networks is a major driver of cell fate decision during embryonic development.

KW - TRANSCRIPTION FACTORS

KW - SUMOYLATION

KW - IDENTIFICATION

KW - INSIGHTS

KW - PATHWAY

KW - COMPLEX

KW - BINDING

U2 - 10.1016/j.celrep.2020.108146

DO - 10.1016/j.celrep.2020.108146

M3 - Journal article

C2 - 32937131

VL - 32

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 11

M1 - 108146

ER -

ID: 250123296