Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment. / Olsen, Nikoline S.; Forero-Junco, Laura; Kot, Witold; Hansen, Lars H.

In: Viruses, Vol. 12, No. 9, 986, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olsen, NS, Forero-Junco, L, Kot, W & Hansen, LH 2020, 'Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment', Viruses, vol. 12, no. 9, 986. https://doi.org/10.3390/v12090986

APA

Olsen, N. S., Forero-Junco, L., Kot, W., & Hansen, L. H. (2020). Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment. Viruses, 12(9), [986]. https://doi.org/10.3390/v12090986

Vancouver

Olsen NS, Forero-Junco L, Kot W, Hansen LH. Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment. Viruses. 2020;12(9). 986. https://doi.org/10.3390/v12090986

Author

Olsen, Nikoline S. ; Forero-Junco, Laura ; Kot, Witold ; Hansen, Lars H. / Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment. In: Viruses. 2020 ; Vol. 12, No. 9.

Bibtex

@article{d043cecb7fbc41909cb8ba4d61d6acec,
title = "Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment",
abstract = "Phages drive bacterial diversity, profoundly influencing microbial communities, from microbiomes to the drivers of global biogeochemical cycling. Aiming to broaden our understanding ofEscherichia coli(MG1655, K-12) phages, we screened 188 Danish wastewater samples and isolated 136 phages. Ninety-two of these have genomic sequences with less than 95% similarity to known phages, while most map to existing genera several represent novel lineages. The isolated phages are highly diverse, estimated to represent roughly one-third of the true diversity of culturable virulent dsDNAEscherichiaphages in Danish wastewater, yet almost half (40%) are not represented in metagenomic databases, emphasising the importance of isolating phages to uncover diversity. Seven viral families,Myoviridae,Siphoviridae,Podoviridae, Drexlerviridae, Chaseviridae, Autographviridae,andMicroviridae,are represented in the dataset. Their genomes vary drastically in length from 5.3 kb to 170.8 kb, with a guanine and cytosine (GC) content ranging from 35.3% to 60.0%. Hence, even for a model host bacterium, substantial diversity remains to be uncovered. These results expand and underline the range of coliphage diversity and demonstrate how far we are from fully disclosing phage diversity and ecology.",
keywords = "bacteriophage, wastewater, Escherichia coli, diversity, genomics, taxonomy, coliphage, COMPLETE GENOME SEQUENCE, PHAGE, BACTERIAL, TAXONOMY, DATABASE, VIRUSES, DNA, BACTERIOPHAGES, EXTRAPOLATION, RAREFACTION",
author = "Olsen, {Nikoline S.} and Laura Forero-Junco and Witold Kot and Hansen, {Lars H.}",
year = "2020",
doi = "10.3390/v12090986",
language = "English",
volume = "12",
journal = "Viruses",
issn = "1999-4915",
publisher = "M D P I AG",
number = "9",

}

RIS

TY - JOUR

T1 - Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment

AU - Olsen, Nikoline S.

AU - Forero-Junco, Laura

AU - Kot, Witold

AU - Hansen, Lars H.

PY - 2020

Y1 - 2020

N2 - Phages drive bacterial diversity, profoundly influencing microbial communities, from microbiomes to the drivers of global biogeochemical cycling. Aiming to broaden our understanding ofEscherichia coli(MG1655, K-12) phages, we screened 188 Danish wastewater samples and isolated 136 phages. Ninety-two of these have genomic sequences with less than 95% similarity to known phages, while most map to existing genera several represent novel lineages. The isolated phages are highly diverse, estimated to represent roughly one-third of the true diversity of culturable virulent dsDNAEscherichiaphages in Danish wastewater, yet almost half (40%) are not represented in metagenomic databases, emphasising the importance of isolating phages to uncover diversity. Seven viral families,Myoviridae,Siphoviridae,Podoviridae, Drexlerviridae, Chaseviridae, Autographviridae,andMicroviridae,are represented in the dataset. Their genomes vary drastically in length from 5.3 kb to 170.8 kb, with a guanine and cytosine (GC) content ranging from 35.3% to 60.0%. Hence, even for a model host bacterium, substantial diversity remains to be uncovered. These results expand and underline the range of coliphage diversity and demonstrate how far we are from fully disclosing phage diversity and ecology.

AB - Phages drive bacterial diversity, profoundly influencing microbial communities, from microbiomes to the drivers of global biogeochemical cycling. Aiming to broaden our understanding ofEscherichia coli(MG1655, K-12) phages, we screened 188 Danish wastewater samples and isolated 136 phages. Ninety-two of these have genomic sequences with less than 95% similarity to known phages, while most map to existing genera several represent novel lineages. The isolated phages are highly diverse, estimated to represent roughly one-third of the true diversity of culturable virulent dsDNAEscherichiaphages in Danish wastewater, yet almost half (40%) are not represented in metagenomic databases, emphasising the importance of isolating phages to uncover diversity. Seven viral families,Myoviridae,Siphoviridae,Podoviridae, Drexlerviridae, Chaseviridae, Autographviridae,andMicroviridae,are represented in the dataset. Their genomes vary drastically in length from 5.3 kb to 170.8 kb, with a guanine and cytosine (GC) content ranging from 35.3% to 60.0%. Hence, even for a model host bacterium, substantial diversity remains to be uncovered. These results expand and underline the range of coliphage diversity and demonstrate how far we are from fully disclosing phage diversity and ecology.

KW - bacteriophage

KW - wastewater

KW - Escherichia coli

KW - diversity

KW - genomics

KW - taxonomy

KW - coliphage

KW - COMPLETE GENOME SEQUENCE

KW - PHAGE

KW - BACTERIAL

KW - TAXONOMY

KW - DATABASE

KW - VIRUSES

KW - DNA

KW - BACTERIOPHAGES

KW - EXTRAPOLATION

KW - RAREFACTION

U2 - 10.3390/v12090986

DO - 10.3390/v12090986

M3 - Journal article

C2 - 32899836

VL - 12

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 9

M1 - 986

ER -

ID: 250917462