The pathogenicity of Bacillus licheniformis was assessed in normal and immunodepressed BALB/c mice. The animals were challenged intravenously with 4 times 10⁷ colony forming units of B. licheniformis (ATCC 14580) and both normal and immunodepressed mice were susceptible. However, the infection was more severe in the immunosuppressed animals. In normal mice, lesions were restricted to the liver and kidneys, while lesions also occurred in other organs of immunodepressed mice. By crossed immunoelectrophoresis it was shown that antigens of B. licheniformis are potent immunogens, and the bacteria could be identified in tissue sections by immunostaining. Immunohistochemically, B. licheniformis was demonstrated in hepatic and pulmonic macrophages, and from some animals the bacteria were also reisolated.