Exercise regulates Akt and glycogen synthase kinase-3 activities in human skeletal muscle
Research output: Contribution to journal › Journal article › Research › peer-review
Activation of Akt and deactivation of GSK3 are critical signals regulating a number of cellular processes in multiple systems. Whether physical exercise alters Akt and GSK3 activity in human skeletal muscle is controversial. β-Catenin, a GSK3 substrate and important Wnt signaling protein that alters gene transcription, has not been investigated in human skeletal muscle. In the present study, eight healthy human subjects performed 30min of cycling exercise at 75% of maximum workload (submaximal) followed by 6 bouts of 60s at 125% maximum workload (maximal). Biopsies of vastus lateralis muscle were taken at rest (basal), and within 15s following cessation of the submaximal and maximal exercise bouts. Exercise at both submaximal and maximal intensities significantly increased Akt activity (40% and 110%, respectively). Increases in Akt activity were accompanied by increases in Akt Thr308 and Ser 473 phosphorylation, decreased GSK3α activity (∼30% at both intensities), and increased phosphorylation of GSK3α Ser21. Exercise at both intensities also decreased β-catenin Ser 33/37Thr41 phosphorylation (50-60% at both intensities). These results demonstrate that Akt, GSK3, and β-catenin signaling are regulated by exercise in human skeletal muscle, and as such identify them as possible molecular mediators of exercise's effect on metabolic and transcriptional processes in skeletal muscle.
Original language | English |
---|---|
Journal | Biochemical and Biophysical Research Communications |
Volume | 319 |
Issue number | 2 |
Pages (from-to) | 419-425 |
Number of pages | 7 |
ISSN | 0006-291X |
DOIs | |
Publication status | Published - 25 Jun 2004 |
Externally published | Yes |
- Adaptation, Akt, Contraction, Exercise, Glycogen synthase, Glycogen synthase kinase-3, Human, Protein kinase, Signaling, Skeletal muscle
Research areas
ID: 239777521