Evidence for multiple recombination events within foot-and-mouth disease viruses circulating in West Eurasia
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Evidence for multiple recombination events within foot-and-mouth disease viruses circulating in West Eurasia. / Jamal, Syed M.; Nazem Shirazi, Mohamad Hossein; Ozyoruk, Fuat; Parlak, Unal; Normann, Preben; Belsham, Graham J.
In: Transboundary and Emerging Diseases, Vol. 67, No. 2, 2020, p. 979-993.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Evidence for multiple recombination events within foot-and-mouth disease viruses circulating in West Eurasia
AU - Jamal, Syed M.
AU - Nazem Shirazi, Mohamad Hossein
AU - Ozyoruk, Fuat
AU - Parlak, Unal
AU - Normann, Preben
AU - Belsham, Graham J.
PY - 2020
Y1 - 2020
N2 - Phylogenetic studies on foot-and-mouth disease viruses (FMDVs) circulating in the West Eurasian region have largely focused on the genomic sequences encoding the structural proteins that determine the serotype. The present study has compared near-complete genome sequences of FMDVs representative of the viruses that circulate in this region. The near-complete genome sequences (ca. 7,600 nt) were generated from multiple overlapping RT-PCR products. These amplicons were from FMDVs belonging to serotypes O, A and Asia-1, including members of the O-PanAsia-II and the A-Iran05 lineages, and of Group-II and Group-VII (Sindh-08) within serotype Asia-1, which are currently predominant and widespread in West Eurasia. These new sequences were analysed together with other sequences obtained from GenBank. Comparison of different regions of the FMDVs genomes revealed evidence for multiple, inter-serotypic, recombination events between FMDVs belonging to the serotypes O, A and Asia-1. It is concluded from the present study that dramatic changes in virus sequences can occur in the field through recombination between different FMDV genomes. These analyses provide information about the ancestry of the serotype O, A and Asia-1 FMDVs that are currently circulating within the West Eurasian region.
AB - Phylogenetic studies on foot-and-mouth disease viruses (FMDVs) circulating in the West Eurasian region have largely focused on the genomic sequences encoding the structural proteins that determine the serotype. The present study has compared near-complete genome sequences of FMDVs representative of the viruses that circulate in this region. The near-complete genome sequences (ca. 7,600 nt) were generated from multiple overlapping RT-PCR products. These amplicons were from FMDVs belonging to serotypes O, A and Asia-1, including members of the O-PanAsia-II and the A-Iran05 lineages, and of Group-II and Group-VII (Sindh-08) within serotype Asia-1, which are currently predominant and widespread in West Eurasia. These new sequences were analysed together with other sequences obtained from GenBank. Comparison of different regions of the FMDVs genomes revealed evidence for multiple, inter-serotypic, recombination events between FMDVs belonging to the serotypes O, A and Asia-1. It is concluded from the present study that dramatic changes in virus sequences can occur in the field through recombination between different FMDV genomes. These analyses provide information about the ancestry of the serotype O, A and Asia-1 FMDVs that are currently circulating within the West Eurasian region.
KW - foot-and-mouth disease virus
KW - mosaic genome
KW - picornavirus
KW - RNA
U2 - 10.1111/tbed.13433
DO - 10.1111/tbed.13433
M3 - Journal article
C2 - 31758840
AN - SCOPUS:85077163930
VL - 67
SP - 979
EP - 993
JO - Transboundary and Emerging Diseases
JF - Transboundary and Emerging Diseases
SN - 1865-1674
IS - 2
ER -
ID: 234276051