Epidermal T cell subsets—Effect of age and antigen exposure in humans and mice
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Epidermal T cell subsets—Effect of age and antigen exposure in humans and mice. / Gadsbøll, Anne Sofie Østergaard; Jee, Mia Hamilton; Ahlström, Malin Glindvad; Dyring-Andersen, Beatrice; Woetmann, Anders; Ødum, Niels; Johansen, Jeanne Duus; Geisler, Carsten; Bonefeld, Charlotte Menné.
In: Contact Dermatitis, Vol. 84, No. 6, 2021, p. 375-384.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Epidermal T cell subsets—Effect of age and antigen exposure in humans and mice
AU - Gadsbøll, Anne Sofie Østergaard
AU - Jee, Mia Hamilton
AU - Ahlström, Malin Glindvad
AU - Dyring-Andersen, Beatrice
AU - Woetmann, Anders
AU - Ødum, Niels
AU - Johansen, Jeanne Duus
AU - Geisler, Carsten
AU - Bonefeld, Charlotte Menné
PY - 2021
Y1 - 2021
N2 - Background: Epidermal T cells play a central role in immune surveillance and in inflammatory skin diseases. Major differences in the epidermal T cell composition are found between adult humans and antigen-inexperienced laboratory mice. Whether this is due to inborn species differences, to different environmental exposures, or a combination of the two is a matter of debate. Objectives: To investigate the role of age and exposure to antigens on epidermal T cell subsets in human and mouse skin. Methods: We isolated T cells from the epidermis from 19 infants and 26 adults, and determined the frequency of CD4+ and CD8+ αβ T cells and γδ T cells by flow cytometry. In addition, we determined the epidermal T cell composition in antigen-inexperienced and antigen-experienced mice. Results: We found that humans are born with very few epidermal T cells. The number increases and the composition changes with age. In antigen-inexperienced mice, the epidermal T cell composition is unaffected by age, but it is dramatically affected by antigen exposure. Conclusion: Taken together, we show that antigen exposure, as opposed to age, is the major factor determining the composition of epidermal T cells, suggesting that the skin of antigen-experienced mice better reflects the immunological conditions in human skin.
AB - Background: Epidermal T cells play a central role in immune surveillance and in inflammatory skin diseases. Major differences in the epidermal T cell composition are found between adult humans and antigen-inexperienced laboratory mice. Whether this is due to inborn species differences, to different environmental exposures, or a combination of the two is a matter of debate. Objectives: To investigate the role of age and exposure to antigens on epidermal T cell subsets in human and mouse skin. Methods: We isolated T cells from the epidermis from 19 infants and 26 adults, and determined the frequency of CD4+ and CD8+ αβ T cells and γδ T cells by flow cytometry. In addition, we determined the epidermal T cell composition in antigen-inexperienced and antigen-experienced mice. Results: We found that humans are born with very few epidermal T cells. The number increases and the composition changes with age. In antigen-inexperienced mice, the epidermal T cell composition is unaffected by age, but it is dramatically affected by antigen exposure. Conclusion: Taken together, we show that antigen exposure, as opposed to age, is the major factor determining the composition of epidermal T cells, suggesting that the skin of antigen-experienced mice better reflects the immunological conditions in human skin.
KW - antigen, contact allergen, epidermis, T cells
U2 - 10.1111/cod.13806
DO - 10.1111/cod.13806
M3 - Journal article
C2 - 33576047
AN - SCOPUS:85102572391
VL - 84
SP - 375
EP - 384
JO - Contact Dermatitis
JF - Contact Dermatitis
SN - 0105-1873
IS - 6
ER -
ID: 258896828