Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy

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Standard

Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy. / Ramos, Cíntia Lacerda; Thorsen, Line; Ryssel, Mia; Nielsen, Dennis Sandris; Siegumfeldt, Henrik; Schwan, Rosane Freitas; Jespersen, Lene.

In: Research in Microbiology, Vol. 165, No. 3, 2014, p. 215-225.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ramos, CL, Thorsen, L, Ryssel, M, Nielsen, DS, Siegumfeldt, H, Schwan, RF & Jespersen, L 2014, 'Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy', Research in Microbiology, vol. 165, no. 3, pp. 215-225. https://doi.org/10.1016/j.resmic.2014.02.005

APA

Ramos, C. L., Thorsen, L., Ryssel, M., Nielsen, D. S., Siegumfeldt, H., Schwan, R. F., & Jespersen, L. (2014). Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy. Research in Microbiology, 165(3), 215-225. https://doi.org/10.1016/j.resmic.2014.02.005

Vancouver

Ramos CL, Thorsen L, Ryssel M, Nielsen DS, Siegumfeldt H, Schwan RF et al. Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy. Research in Microbiology. 2014;165(3):215-225. https://doi.org/10.1016/j.resmic.2014.02.005

Author

Ramos, Cíntia Lacerda ; Thorsen, Line ; Ryssel, Mia ; Nielsen, Dennis Sandris ; Siegumfeldt, Henrik ; Schwan, Rosane Freitas ; Jespersen, Lene. / Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy. In: Research in Microbiology. 2014 ; Vol. 165, No. 3. pp. 215-225.

Bibtex

@article{7ffcd80a21d7408aa6e57ff0d6d006f5,
title = "Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy",
abstract = "In the present work, an in vitro model of the gastrointestinal tract (GIT) was developed to obtain real-time observations of the pH homeostasis of single cells of probiotic Lactobacillus spp. strains as a measure of their physiological state. Changes in the intracellular pH (pHi) were determined using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with pHi values ranging from 6.5 to 7.5, 3.5 to 5.6 and 6.5 to 8.0 or higher during passage of saliva (pH 6.4), gastric (pH 3.5) and intestinal juices (pH 6.4), respectively. When nutrients were added to gastric juice, the isolate L. brevis significantly decreased its pHi closer to the extracellular pH (pHex) than in gastric juice without nutrients. This was not the case for L. plantarum. This study is the first to produce an in vitro GIT model enabling real-time monitoring of pH homeostasis of single cells in response to the wide range of pHex of the GIT. Furthermore, it was possible to observe the heterogeneous response of single cells. The technique can be used to determine the survival and physiological conditions of potential probiotics and other microorganisms during passage through the GIT.",
author = "Ramos, {C{\'i}ntia Lacerda} and Line Thorsen and Mia Ryssel and Nielsen, {Dennis Sandris} and Henrik Siegumfeldt and Schwan, {Rosane Freitas} and Lene Jespersen",
note = "Copyright {\textcopyright} 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.",
year = "2014",
doi = "10.1016/j.resmic.2014.02.005",
language = "English",
volume = "165",
pages = "215--225",
journal = "Research in Microbiology",
issn = "0923-2508",
publisher = "Elsevier Masson",
number = "3",

}

RIS

TY - JOUR

T1 - Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy

AU - Ramos, Cíntia Lacerda

AU - Thorsen, Line

AU - Ryssel, Mia

AU - Nielsen, Dennis Sandris

AU - Siegumfeldt, Henrik

AU - Schwan, Rosane Freitas

AU - Jespersen, Lene

N1 - Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

PY - 2014

Y1 - 2014

N2 - In the present work, an in vitro model of the gastrointestinal tract (GIT) was developed to obtain real-time observations of the pH homeostasis of single cells of probiotic Lactobacillus spp. strains as a measure of their physiological state. Changes in the intracellular pH (pHi) were determined using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with pHi values ranging from 6.5 to 7.5, 3.5 to 5.6 and 6.5 to 8.0 or higher during passage of saliva (pH 6.4), gastric (pH 3.5) and intestinal juices (pH 6.4), respectively. When nutrients were added to gastric juice, the isolate L. brevis significantly decreased its pHi closer to the extracellular pH (pHex) than in gastric juice without nutrients. This was not the case for L. plantarum. This study is the first to produce an in vitro GIT model enabling real-time monitoring of pH homeostasis of single cells in response to the wide range of pHex of the GIT. Furthermore, it was possible to observe the heterogeneous response of single cells. The technique can be used to determine the survival and physiological conditions of potential probiotics and other microorganisms during passage through the GIT.

AB - In the present work, an in vitro model of the gastrointestinal tract (GIT) was developed to obtain real-time observations of the pH homeostasis of single cells of probiotic Lactobacillus spp. strains as a measure of their physiological state. Changes in the intracellular pH (pHi) were determined using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with pHi values ranging from 6.5 to 7.5, 3.5 to 5.6 and 6.5 to 8.0 or higher during passage of saliva (pH 6.4), gastric (pH 3.5) and intestinal juices (pH 6.4), respectively. When nutrients were added to gastric juice, the isolate L. brevis significantly decreased its pHi closer to the extracellular pH (pHex) than in gastric juice without nutrients. This was not the case for L. plantarum. This study is the first to produce an in vitro GIT model enabling real-time monitoring of pH homeostasis of single cells in response to the wide range of pHex of the GIT. Furthermore, it was possible to observe the heterogeneous response of single cells. The technique can be used to determine the survival and physiological conditions of potential probiotics and other microorganisms during passage through the GIT.

U2 - 10.1016/j.resmic.2014.02.005

DO - 10.1016/j.resmic.2014.02.005

M3 - Journal article

C2 - 24607712

VL - 165

SP - 215

EP - 225

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

IS - 3

ER -

ID: 106412584