TY - JOUR

T1 - Early osteoarthritis and microdialysis

T2 - a novel in vivo approach for measurements of biochemical markers in the perisynovium and intraarticularly

AU - Helmark, Ida Carøe

AU - Mikkelsen, U R

AU - Krogsgaard, M R

AU - Belhage, B

AU - Petersen, M C H

AU - Langberg, Henning

AU - Kjaer, M

PY - 2010

Y1 - 2010

N2 - The microdialysis technique was evaluated as a possible method to obtain local measurements of biochemical markers from knee joints with degenerative changes. Seven patients scheduled for arthroscopy of the knee due to minor to moderate degenerative changes had microdialysis catheters inserted under ultrasonographic guidance, intraarticularly and in the synovium-close tissue. Catheters were perfused at a rate of 2 µl/min for approximately 100 min with a Ringer solution containing radioactively labeled glucose, and the positions of the catheters were later visualized during arthroscopy. All intraarticular catheters and 6/7 subsynovial catheters were positioned correctly. Relative recovery (RR) was intraarticularly 0.64 ± 0.02 (mean ± SEM) and synovium-close 0.54 ± 0.06. Mean values of cartilage oligomeric matrix protein (COMP), aggrecan and glucosyl-galactosyl-pyridinoline in the intraarticular dialysates were 18.1 ± 7.0 U/l, 243.6 ± 108.6 ng/ml and 108.0 ± 29.0 pmol/ml, respectively. COMP and glucosyl-galactosyl-pyridinoline concentrations were significantly higher intraarticularly compared to perisynovial tissue (P <0.05), whereas for aggrecan, no significant difference was found (P = 0.06). The microdialysis method can be used for intraarticular and subsynovial determination of metabolites in human knees at these sites. The present methodology displays a potential for future studies of simultaneous biochemical changes within and around joints.

AB - The microdialysis technique was evaluated as a possible method to obtain local measurements of biochemical markers from knee joints with degenerative changes. Seven patients scheduled for arthroscopy of the knee due to minor to moderate degenerative changes had microdialysis catheters inserted under ultrasonographic guidance, intraarticularly and in the synovium-close tissue. Catheters were perfused at a rate of 2 µl/min for approximately 100 min with a Ringer solution containing radioactively labeled glucose, and the positions of the catheters were later visualized during arthroscopy. All intraarticular catheters and 6/7 subsynovial catheters were positioned correctly. Relative recovery (RR) was intraarticularly 0.64 ± 0.02 (mean ± SEM) and synovium-close 0.54 ± 0.06. Mean values of cartilage oligomeric matrix protein (COMP), aggrecan and glucosyl-galactosyl-pyridinoline in the intraarticular dialysates were 18.1 ± 7.0 U/l, 243.6 ± 108.6 ng/ml and 108.0 ± 29.0 pmol/ml, respectively. COMP and glucosyl-galactosyl-pyridinoline concentrations were significantly higher intraarticularly compared to perisynovial tissue (P <0.05), whereas for aggrecan, no significant difference was found (P = 0.06). The microdialysis method can be used for intraarticular and subsynovial determination of metabolites in human knees at these sites. The present methodology displays a potential for future studies of simultaneous biochemical changes within and around joints.

KW - Adult

KW - Aged

KW - Aggrecans

KW - Amino Acids

KW - Arthroscopy

KW - Biological Markers

KW - Catheters

KW - Early Diagnosis

KW - Enzyme-Linked Immunosorbent Assay

KW - Extracellular Matrix Proteins

KW - Female

KW - Glycoproteins

KW - Humans

KW - Knee Joint

KW - Male

KW - Microdialysis

KW - Middle Aged

KW - Osteoarthritis, Knee

KW - Sampling Studies

KW - Sensitivity and Specificity

KW - Severity of Illness Index

KW - Synovial Membrane

U2 - 10.1007/s00167-010-1115-3

DO - 10.1007/s00167-010-1115-3

M3 - Journal article

C2 - 20405106

VL - 18

SP - 1617

EP - 1623

JO - Knee Surgery, Sports Traumatology, Arthroscopy

JF - Knee Surgery, Sports Traumatology, Arthroscopy

SN - 0942-2056

IS - 11

ER -