Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma. / Rossing, Maria; Helweg-Larsen, Rehannah Borup; Henao Giraldo, Ricardo; Winther, Ole; Vikeså, Jonas; Niazi, Omid; Godballe, Christian; Krogdahl, Annelise; Glud, Martin; Sørensen, Christian Hjort; Kiss, Katalin; Bennedbæk, Finn Noe; Nielsen, Finn Cilius.

In: Journal of Molecular Endocrinology, Vol. 48, No. 1, 2012, p. 11-23.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rossing, M, Helweg-Larsen, RB, Henao Giraldo, R, Winther, O, Vikeså, J, Niazi, O, Godballe, C, Krogdahl, A, Glud, M, Sørensen, CH, Kiss, K, Bennedbæk, FN & Nielsen, FC 2012, 'Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma', Journal of Molecular Endocrinology, vol. 48, no. 1, pp. 11-23. https://doi.org/10.1530/JME-11-0039

APA

Rossing, M., Helweg-Larsen, R. B., Henao Giraldo, R., Winther, O., Vikeså, J., Niazi, O., ... Nielsen, F. C. (2012). Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma. Journal of Molecular Endocrinology, 48(1), 11-23. https://doi.org/10.1530/JME-11-0039

Vancouver

Rossing M, Helweg-Larsen RB, Henao Giraldo R, Winther O, Vikeså J, Niazi O et al. Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma. Journal of Molecular Endocrinology. 2012;48(1):11-23. https://doi.org/10.1530/JME-11-0039

Author

Rossing, Maria ; Helweg-Larsen, Rehannah Borup ; Henao Giraldo, Ricardo ; Winther, Ole ; Vikeså, Jonas ; Niazi, Omid ; Godballe, Christian ; Krogdahl, Annelise ; Glud, Martin ; Sørensen, Christian Hjort ; Kiss, Katalin ; Bennedbæk, Finn Noe ; Nielsen, Finn Cilius. / Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma. In: Journal of Molecular Endocrinology. 2012 ; Vol. 48, No. 1. pp. 11-23.

Bibtex

@article{9ff332e939704b90bbfb6d5bf1b9897e,
title = "Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma",
abstract = "The molecular determinants of thyroid follicular nodules are incompletely understood and assessment of malignancy is a diagnostic challenge. Since microRNA (miRNA) analyses could provide new leads to malignant progression, we characterized the global miRNA expression in follicular adenoma (FA) and follicular carcinoma (FC). Comparison of carcinoma and adenoma with normal thyroid revealed 150 and 107 differentially expressed miRNAs. Most miRNAs were down-regulated and especially miR-199b-5p and miR-144 which were essentially lost in the carcinomas. Integration of the changed miRNAs with differentially expressed mRNAs demonstrated an enrichment of seed-sites among up-regulated transcripts encoding proteins implicated in thyroid tumourigenesis. This was substantiated by the demonstration that pre-miR-199b reduced proliferation when added to cultured follicular thyroid carcinoma cells. The down-regulated miRNAs in FC exhibited a substantial similarity with down-regulated miRNAs in anaplastic carcinoma and by gene set enrichment analysis, we observed a significant identity between target mRNAs in FC and transcripts up-regulated in anaplastic carcinoma. To examine the diagnostic potential of miRNA expression pattern in distinguishing malignant from benign nodules we employed a supervised learning algorithm and leave-one-out-cross-validation. By this procedure, FA and FC were identified with a negative predicted value (NPV) of 83{\%} (data generated by microarray platform) and of 92{\%} (data generated by qRT-PCR platform). We conclude that follicular neoplasia is associated by major changes in miRNA expression that may promote malignant transformation by increasing the expression of transcripts encoding tumourigenic factors. Moreover, miRNA profiling may facilitate the diagnosis of carcinoma vs. adenoma.",
author = "Maria Rossing and Helweg-Larsen, {Rehannah Borup} and {Henao Giraldo}, Ricardo and Ole Winther and Jonas Vikes{\aa} and Omid Niazi and Christian Godballe and Annelise Krogdahl and Martin Glud and S{\o}rensen, {Christian Hjort} and Katalin Kiss and Bennedb{\ae}k, {Finn Noe} and Nielsen, {Finn Cilius}",
year = "2012",
doi = "10.1530/JME-11-0039",
language = "English",
volume = "48",
pages = "11--23",
journal = "Journal of Molecular Endocrinology",
issn = "0952-5041",
publisher = "BioScientifica Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Down-regulation of microRNAs controlling tumourigenic factors in follicular thyroid carcinoma

AU - Rossing, Maria

AU - Helweg-Larsen, Rehannah Borup

AU - Henao Giraldo, Ricardo

AU - Winther, Ole

AU - Vikeså, Jonas

AU - Niazi, Omid

AU - Godballe, Christian

AU - Krogdahl, Annelise

AU - Glud, Martin

AU - Sørensen, Christian Hjort

AU - Kiss, Katalin

AU - Bennedbæk, Finn Noe

AU - Nielsen, Finn Cilius

PY - 2012

Y1 - 2012

N2 - The molecular determinants of thyroid follicular nodules are incompletely understood and assessment of malignancy is a diagnostic challenge. Since microRNA (miRNA) analyses could provide new leads to malignant progression, we characterized the global miRNA expression in follicular adenoma (FA) and follicular carcinoma (FC). Comparison of carcinoma and adenoma with normal thyroid revealed 150 and 107 differentially expressed miRNAs. Most miRNAs were down-regulated and especially miR-199b-5p and miR-144 which were essentially lost in the carcinomas. Integration of the changed miRNAs with differentially expressed mRNAs demonstrated an enrichment of seed-sites among up-regulated transcripts encoding proteins implicated in thyroid tumourigenesis. This was substantiated by the demonstration that pre-miR-199b reduced proliferation when added to cultured follicular thyroid carcinoma cells. The down-regulated miRNAs in FC exhibited a substantial similarity with down-regulated miRNAs in anaplastic carcinoma and by gene set enrichment analysis, we observed a significant identity between target mRNAs in FC and transcripts up-regulated in anaplastic carcinoma. To examine the diagnostic potential of miRNA expression pattern in distinguishing malignant from benign nodules we employed a supervised learning algorithm and leave-one-out-cross-validation. By this procedure, FA and FC were identified with a negative predicted value (NPV) of 83% (data generated by microarray platform) and of 92% (data generated by qRT-PCR platform). We conclude that follicular neoplasia is associated by major changes in miRNA expression that may promote malignant transformation by increasing the expression of transcripts encoding tumourigenic factors. Moreover, miRNA profiling may facilitate the diagnosis of carcinoma vs. adenoma.

AB - The molecular determinants of thyroid follicular nodules are incompletely understood and assessment of malignancy is a diagnostic challenge. Since microRNA (miRNA) analyses could provide new leads to malignant progression, we characterized the global miRNA expression in follicular adenoma (FA) and follicular carcinoma (FC). Comparison of carcinoma and adenoma with normal thyroid revealed 150 and 107 differentially expressed miRNAs. Most miRNAs were down-regulated and especially miR-199b-5p and miR-144 which were essentially lost in the carcinomas. Integration of the changed miRNAs with differentially expressed mRNAs demonstrated an enrichment of seed-sites among up-regulated transcripts encoding proteins implicated in thyroid tumourigenesis. This was substantiated by the demonstration that pre-miR-199b reduced proliferation when added to cultured follicular thyroid carcinoma cells. The down-regulated miRNAs in FC exhibited a substantial similarity with down-regulated miRNAs in anaplastic carcinoma and by gene set enrichment analysis, we observed a significant identity between target mRNAs in FC and transcripts up-regulated in anaplastic carcinoma. To examine the diagnostic potential of miRNA expression pattern in distinguishing malignant from benign nodules we employed a supervised learning algorithm and leave-one-out-cross-validation. By this procedure, FA and FC were identified with a negative predicted value (NPV) of 83% (data generated by microarray platform) and of 92% (data generated by qRT-PCR platform). We conclude that follicular neoplasia is associated by major changes in miRNA expression that may promote malignant transformation by increasing the expression of transcripts encoding tumourigenic factors. Moreover, miRNA profiling may facilitate the diagnosis of carcinoma vs. adenoma.

U2 - 10.1530/JME-11-0039

DO - 10.1530/JME-11-0039

M3 - Journal article

C2 - 22049245

VL - 48

SP - 11

EP - 23

JO - Journal of Molecular Endocrinology

JF - Journal of Molecular Endocrinology

SN - 0952-5041

IS - 1

ER -

ID: 40143826