DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch. / Norregaard, Kamilla; Andersson, Magnus; Sneppen, Kim; Nielsen, Peter Eigil; Brown, Stanley; Oddershede, Lene B.

In: Proceedings of the National Academy of Sciences USA (PNAS), Vol. 110, No. 43, 22.10.2013, p. 17386-91.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Norregaard, K, Andersson, M, Sneppen, K, Nielsen, PE, Brown, S & Oddershede, LB 2013, 'DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch', Proceedings of the National Academy of Sciences USA (PNAS), vol. 110, no. 43, pp. 17386-91. https://doi.org/10.1073/pnas.1215907110

APA

Norregaard, K., Andersson, M., Sneppen, K., Nielsen, P. E., Brown, S., & Oddershede, L. B. (2013). DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch. Proceedings of the National Academy of Sciences USA (PNAS), 110(43), 17386-91. https://doi.org/10.1073/pnas.1215907110

Vancouver

Norregaard K, Andersson M, Sneppen K, Nielsen PE, Brown S, Oddershede LB. DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch. Proceedings of the National Academy of Sciences USA (PNAS). 2013 Oct 22;110(43):17386-91. https://doi.org/10.1073/pnas.1215907110

Author

Norregaard, Kamilla ; Andersson, Magnus ; Sneppen, Kim ; Nielsen, Peter Eigil ; Brown, Stanley ; Oddershede, Lene B. / DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch. In: Proceedings of the National Academy of Sciences USA (PNAS). 2013 ; Vol. 110, No. 43. pp. 17386-91.

Bibtex

@article{6be20c0ce60b40dc980aad31041ef6da,
title = "DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch",
abstract = "Bacteriophage λ stably maintains its dormant prophage state but efficiently enters lytic development in response to DNA damage. The mediator of these processes is the λ repressor protein, CI, and its interactions with λ operator DNA. This λ switch is a model on the basis of which epigenetic switch regulation is understood. Using single molecule analysis, we directly examined the stability of the CI-operator structure in its natural, supercoiled state. We marked positions adjacent to the λ operators with peptide nucleic acids and monitored their movement by tethered particle tracking. Compared with relaxed DNA, the presence of supercoils greatly enhances juxtaposition probability. Also, the efficiency and cooperativity of the λ switch is significantly increased in the supercoiled system compared with a linear assay, increasing the Hill coefficient.",
keywords = "Algorithms, Bacteriophage lambda, DNA, Superhelical, DNA, Viral, Epigenesis, Genetic, Lysogeny, Models, Genetic, Operator Regions, Genetic, Prophages, Protein Binding, Repressor Proteins, Thermodynamics, Viral Regulatory and Accessory Proteins, Virus Activation",
author = "Kamilla Norregaard and Magnus Andersson and Kim Sneppen and Nielsen, {Peter Eigil} and Stanley Brown and Oddershede, {Lene B}",
year = "2013",
month = oct,
day = "22",
doi = "10.1073/pnas.1215907110",
language = "English",
volume = "110",
pages = "17386--91",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "43",

}

RIS

TY - JOUR

T1 - DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch

AU - Norregaard, Kamilla

AU - Andersson, Magnus

AU - Sneppen, Kim

AU - Nielsen, Peter Eigil

AU - Brown, Stanley

AU - Oddershede, Lene B

PY - 2013/10/22

Y1 - 2013/10/22

N2 - Bacteriophage λ stably maintains its dormant prophage state but efficiently enters lytic development in response to DNA damage. The mediator of these processes is the λ repressor protein, CI, and its interactions with λ operator DNA. This λ switch is a model on the basis of which epigenetic switch regulation is understood. Using single molecule analysis, we directly examined the stability of the CI-operator structure in its natural, supercoiled state. We marked positions adjacent to the λ operators with peptide nucleic acids and monitored their movement by tethered particle tracking. Compared with relaxed DNA, the presence of supercoils greatly enhances juxtaposition probability. Also, the efficiency and cooperativity of the λ switch is significantly increased in the supercoiled system compared with a linear assay, increasing the Hill coefficient.

AB - Bacteriophage λ stably maintains its dormant prophage state but efficiently enters lytic development in response to DNA damage. The mediator of these processes is the λ repressor protein, CI, and its interactions with λ operator DNA. This λ switch is a model on the basis of which epigenetic switch regulation is understood. Using single molecule analysis, we directly examined the stability of the CI-operator structure in its natural, supercoiled state. We marked positions adjacent to the λ operators with peptide nucleic acids and monitored their movement by tethered particle tracking. Compared with relaxed DNA, the presence of supercoils greatly enhances juxtaposition probability. Also, the efficiency and cooperativity of the λ switch is significantly increased in the supercoiled system compared with a linear assay, increasing the Hill coefficient.

KW - Algorithms

KW - Bacteriophage lambda

KW - DNA, Superhelical

KW - DNA, Viral

KW - Epigenesis, Genetic

KW - Lysogeny

KW - Models, Genetic

KW - Operator Regions, Genetic

KW - Prophages

KW - Protein Binding

KW - Repressor Proteins

KW - Thermodynamics

KW - Viral Regulatory and Accessory Proteins

KW - Virus Activation

U2 - 10.1073/pnas.1215907110

DO - 10.1073/pnas.1215907110

M3 - Journal article

C2 - 24101469

VL - 110

SP - 17386

EP - 17391

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 43

ER -

ID: 77591206