Distinct narcolepsy syndromes in Orexin receptor-2 and Orexin null mice: molecular genetic dissection of Non-REM and REM sleep regulatory processes
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Distinct narcolepsy syndromes in Orexin receptor-2 and Orexin null mice : molecular genetic dissection of Non-REM and REM sleep regulatory processes. / Willie, Jon T; Chemelli, Richard M; Sinton, Christopher M; Tokita, Shigeru; Williams, S Clay; Kisanuki, Yaz Y; Marcus, Jacob N; Lee, Charlotte; Elmquist, Joel K; Kohlmeier, Kristi Anne; Leonard, Christopher S; Richardson, James A; Hammer, Robert E; Yanagisawa, Masashi.
In: Neuron, Vol. 38, No. 5, 2003, p. 715-30.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Distinct narcolepsy syndromes in Orexin receptor-2 and Orexin null mice
T2 - molecular genetic dissection of Non-REM and REM sleep regulatory processes
AU - Willie, Jon T
AU - Chemelli, Richard M
AU - Sinton, Christopher M
AU - Tokita, Shigeru
AU - Williams, S Clay
AU - Kisanuki, Yaz Y
AU - Marcus, Jacob N
AU - Lee, Charlotte
AU - Elmquist, Joel K
AU - Kohlmeier, Kristi Anne
AU - Leonard, Christopher S
AU - Richardson, James A
AU - Hammer, Robert E
AU - Yanagisawa, Masashi
PY - 2003
Y1 - 2003
N2 - Narcolepsy-cataplexy, a neurological disorder associated with the absence of hypothalamic orexin (hypocretin) neuropeptides, consists of two underlying problems: inability to maintain wakefulness and intrusion of rapid eye movement (REM) sleep into wakefulness. Here we document, using behavioral, electrophysiological, and pharmacological criteria, two distinct classes of behavioral arrests exhibited by mice deficient in orexin-mediated signaling. Both OX2R(-/-) and orexin(-/-) mice are similarly affected with behaviorally abnormal attacks of non-REM sleep ("sleep attacks") and show similar degrees of disrupted wakefulness. In contrast, OX2R(-/-) mice are only mildly affected with cataplexy-like attacks of REM sleep, whereas orexin(-/-) mice are severely affected. Absence of OX2Rs eliminates orexin-evoked excitation of histaminergic neurons in the hypothalamus, which gate non-REM sleep onset. While normal regulation of wake/non-REM sleep transitions depends critically upon OX2R activation, the profound dysregulation of REM sleep control unique to the narcolepsy-cataplexy syndrome emerges from loss of signaling through both OX2R-dependent and OX2R-independent pathways.
AB - Narcolepsy-cataplexy, a neurological disorder associated with the absence of hypothalamic orexin (hypocretin) neuropeptides, consists of two underlying problems: inability to maintain wakefulness and intrusion of rapid eye movement (REM) sleep into wakefulness. Here we document, using behavioral, electrophysiological, and pharmacological criteria, two distinct classes of behavioral arrests exhibited by mice deficient in orexin-mediated signaling. Both OX2R(-/-) and orexin(-/-) mice are similarly affected with behaviorally abnormal attacks of non-REM sleep ("sleep attacks") and show similar degrees of disrupted wakefulness. In contrast, OX2R(-/-) mice are only mildly affected with cataplexy-like attacks of REM sleep, whereas orexin(-/-) mice are severely affected. Absence of OX2Rs eliminates orexin-evoked excitation of histaminergic neurons in the hypothalamus, which gate non-REM sleep onset. While normal regulation of wake/non-REM sleep transitions depends critically upon OX2R activation, the profound dysregulation of REM sleep control unique to the narcolepsy-cataplexy syndrome emerges from loss of signaling through both OX2R-dependent and OX2R-independent pathways.
KW - Animals
KW - Arousal
KW - Carrier Proteins
KW - Cells, Cultured
KW - Clomipramine
KW - Disease Models, Animal
KW - Efferent Pathways
KW - Electroencephalography
KW - Electromyography
KW - Histamine
KW - Hypothalamic Area, Lateral
KW - Hypothalamus
KW - Intracellular Signaling Peptides and Proteins
KW - Male
KW - Mice
KW - Mice, Knockout
KW - Narcolepsy
KW - Neuropeptides
KW - Receptors, G-Protein-Coupled
KW - Receptors, Neuropeptide
KW - Sleep
KW - Sleep, REM
KW - Synaptic Transmission
M3 - Journal article
C2 - 12797957
VL - 38
SP - 715
EP - 730
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 5
ER -
ID: 38346557