Distinct CED-10/Rac1 domains confer context-specific functions in development

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Distinct CED-10/Rac1 domains confer context-specific functions in development. / Nørgaard, Steffen; Deng, Shuer; Cao, Wei; Pocock, Roger.

In: P L o S Genetics, Vol. 14, No. 9, e1007670, 2018, p. 1-24.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nørgaard, S, Deng, S, Cao, W & Pocock, R 2018, 'Distinct CED-10/Rac1 domains confer context-specific functions in development', P L o S Genetics, vol. 14, no. 9, e1007670, pp. 1-24. https://doi.org/10.1371/journal.pgen.1007670

APA

Nørgaard, S., Deng, S., Cao, W., & Pocock, R. (2018). Distinct CED-10/Rac1 domains confer context-specific functions in development. P L o S Genetics, 14(9), 1-24. [e1007670]. https://doi.org/10.1371/journal.pgen.1007670

Vancouver

Nørgaard S, Deng S, Cao W, Pocock R. Distinct CED-10/Rac1 domains confer context-specific functions in development. P L o S Genetics. 2018;14(9):1-24. e1007670. https://doi.org/10.1371/journal.pgen.1007670

Author

Nørgaard, Steffen ; Deng, Shuer ; Cao, Wei ; Pocock, Roger. / Distinct CED-10/Rac1 domains confer context-specific functions in development. In: P L o S Genetics. 2018 ; Vol. 14, No. 9. pp. 1-24.

Bibtex

@article{857dac99e13b49e797f54e4a89957a83,
title = "Distinct CED-10/Rac1 domains confer context-specific functions in development",
abstract = "Rac GTPases act as master switches to coordinate multiple interweaved signaling pathways. A major function for Rac GTPases is to control neurite development by influencing downstream effector molecules and pathways. In Caenorhabditis elegans, the Rac proteins CED-10, RAC-2 and MIG-2 act in parallel to control axon outgrowth and guidance. Here, we have identified a single glycine residue in the CED-10/Rac1 Switch 1 region that confers a non-redundant function in axon outgrowth but not guidance. Mutation of this glycine to glutamic acid (G30E) reduces GTP binding and inhibits axon outgrowth but does not affect other canonical CED-10 functions. This demonstrates previously unappreciated domain-specific functions within the CED-10 protein. Further, we reveal that when CED-10 function is diminished, the adaptor protein NAB-1 (Neurabin) and its interacting partner SYD-1 (Rho-GAP-like protein) can act as inhibitors of axon outgrowth. Together, we reveal that specific domains and residues within Rac GTPases can confer context-dependent functions during animal development.",
author = "Steffen N{\o}rgaard and Shuer Deng and Wei Cao and Roger Pocock",
year = "2018",
doi = "10.1371/journal.pgen.1007670",
language = "English",
volume = "14",
pages = "1--24",
journal = "P L o S Genetics",
issn = "1553-7390",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - Distinct CED-10/Rac1 domains confer context-specific functions in development

AU - Nørgaard, Steffen

AU - Deng, Shuer

AU - Cao, Wei

AU - Pocock, Roger

PY - 2018

Y1 - 2018

N2 - Rac GTPases act as master switches to coordinate multiple interweaved signaling pathways. A major function for Rac GTPases is to control neurite development by influencing downstream effector molecules and pathways. In Caenorhabditis elegans, the Rac proteins CED-10, RAC-2 and MIG-2 act in parallel to control axon outgrowth and guidance. Here, we have identified a single glycine residue in the CED-10/Rac1 Switch 1 region that confers a non-redundant function in axon outgrowth but not guidance. Mutation of this glycine to glutamic acid (G30E) reduces GTP binding and inhibits axon outgrowth but does not affect other canonical CED-10 functions. This demonstrates previously unappreciated domain-specific functions within the CED-10 protein. Further, we reveal that when CED-10 function is diminished, the adaptor protein NAB-1 (Neurabin) and its interacting partner SYD-1 (Rho-GAP-like protein) can act as inhibitors of axon outgrowth. Together, we reveal that specific domains and residues within Rac GTPases can confer context-dependent functions during animal development.

AB - Rac GTPases act as master switches to coordinate multiple interweaved signaling pathways. A major function for Rac GTPases is to control neurite development by influencing downstream effector molecules and pathways. In Caenorhabditis elegans, the Rac proteins CED-10, RAC-2 and MIG-2 act in parallel to control axon outgrowth and guidance. Here, we have identified a single glycine residue in the CED-10/Rac1 Switch 1 region that confers a non-redundant function in axon outgrowth but not guidance. Mutation of this glycine to glutamic acid (G30E) reduces GTP binding and inhibits axon outgrowth but does not affect other canonical CED-10 functions. This demonstrates previously unappreciated domain-specific functions within the CED-10 protein. Further, we reveal that when CED-10 function is diminished, the adaptor protein NAB-1 (Neurabin) and its interacting partner SYD-1 (Rho-GAP-like protein) can act as inhibitors of axon outgrowth. Together, we reveal that specific domains and residues within Rac GTPases can confer context-dependent functions during animal development.

U2 - 10.1371/journal.pgen.1007670

DO - 10.1371/journal.pgen.1007670

M3 - Journal article

C2 - 30265669

AN - SCOPUS:85054589460

VL - 14

SP - 1

EP - 24

JO - P L o S Genetics

JF - P L o S Genetics

SN - 1553-7390

IS - 9

M1 - e1007670

ER -

ID: 204184030