Direct Measurement of Cerebrospinal Fluid Production in Mice
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- Direct Measurement of Cerebrospinal Fluid Production in Mice
Final published version, 2.35 MB, PDF document
The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4th ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-β. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked.
Original language | English |
---|---|
Article number | 108524 |
Journal | Cell Reports |
Volume | 33 |
Issue number | 12 |
Number of pages | 13 |
ISSN | 2211-1247 |
DOIs | |
Publication status | Published - 2020 |
- aging, amyloid-β, glymphatic system, sex difference, sleep-wake cycle
Research areas
Number of downloads are based on statistics from Google Scholar and www.ku.dk
ID: 255098644