Differential expression and co-expression gene networks reveal candidate biomarkers of boar taint in non-castrated pigs
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Differential expression and co-expression gene networks reveal candidate biomarkers of boar taint in non-castrated pigs. / Drag, Markus; Skinkyté-Juskiené, Rúta; Do, Duy Ngoc; Kogelman, Lisette; Kadarmideen, Haja N.
In: Scientific Reports, Vol. 7, 12205, 22.09.2017.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Differential expression and co-expression gene networks reveal candidate biomarkers of boar taint in non-castrated pigs
AU - Drag, Markus
AU - Skinkyté-Juskiené, Rúta
AU - Do, Duy Ngoc
AU - Kogelman, Lisette
AU - Kadarmideen, Haja N
PY - 2017/9/22
Y1 - 2017/9/22
N2 - Boar taint (BT) is an offensive odour or taste observed in pork from a proportion of non-castrated male pigs. Surgical castration is effective in avoiding BT, but animal welfare issues have created an incentive for alternatives such as genomic selection. In order to find candidate biomarkers, gene expression profiles were analysed from tissues of non-castrated pigs grouped by their genetic merit of BT. Differential expression analysis revealed substantial changes with log-transformed fold changes of liver and testis from −3.39 to 2.96 and −7.51 to 3.53, respectively. Co-expression network analysis revealed one module with a correlation of −0.27 in liver and three modules with correlations of 0.31, −0.44 and −0.49 in testis. Differential expression and co-expression analysis revealed candidate biomarkers with varying biological functions: phase I (COQ3, COX6C, CYP2J2, CYP2B6, ACOX2) and phase II metabolism (GSTO1, GSR, FMO3) of skatole and androstenone in liver to steroidgenesis (HSD17B7, HSD17B8, CYP27A1), regulation of steroidgenesis (STARD10, CYB5R3) and GnRH signalling (MAPK3, MAP2K2, MAP3K2) in testis. Overrepresented pathways included “Ribosome”, “Protein export” and “Oxidative phosphorylation” in liver and “Steroid hormone biosynthesis” and “Gap junction” in testis. Future work should evaluate the biomarkers in large populations to ensure their usefulness in genomic selection programs.
AB - Boar taint (BT) is an offensive odour or taste observed in pork from a proportion of non-castrated male pigs. Surgical castration is effective in avoiding BT, but animal welfare issues have created an incentive for alternatives such as genomic selection. In order to find candidate biomarkers, gene expression profiles were analysed from tissues of non-castrated pigs grouped by their genetic merit of BT. Differential expression analysis revealed substantial changes with log-transformed fold changes of liver and testis from −3.39 to 2.96 and −7.51 to 3.53, respectively. Co-expression network analysis revealed one module with a correlation of −0.27 in liver and three modules with correlations of 0.31, −0.44 and −0.49 in testis. Differential expression and co-expression analysis revealed candidate biomarkers with varying biological functions: phase I (COQ3, COX6C, CYP2J2, CYP2B6, ACOX2) and phase II metabolism (GSTO1, GSR, FMO3) of skatole and androstenone in liver to steroidgenesis (HSD17B7, HSD17B8, CYP27A1), regulation of steroidgenesis (STARD10, CYB5R3) and GnRH signalling (MAPK3, MAP2K2, MAP3K2) in testis. Overrepresented pathways included “Ribosome”, “Protein export” and “Oxidative phosphorylation” in liver and “Steroid hormone biosynthesis” and “Gap junction” in testis. Future work should evaluate the biomarkers in large populations to ensure their usefulness in genomic selection programs.
U2 - 10.1038/s41598-017-11928-0
DO - 10.1038/s41598-017-11928-0
M3 - Journal article
C2 - 28939879
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 12205
ER -
ID: 183797749