Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone
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Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone. / Jensen, Marie Aarrebo; Hansen, Åse Marie; Abrahamsson, Peter; Nørgaard, Asger W.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 879, No. 25, 2011, p. 2527-32.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone
AU - Jensen, Marie Aarrebo
AU - Hansen, Åse Marie
AU - Abrahamsson, Peter
AU - Nørgaard, Asger W
N1 - Copyright © 2011 Elsevier B.V. All rights reserved.
PY - 2011
Y1 - 2011
N2 - Circadian disruption can have several possible health consequences, but is not well studied. In order to measure circadian disruption, in relation to shift or night work, we developed a simple and sensitive method for the simultaneous determination of melatonin, cortisol and testosterone in human saliva. We used liquid-liquid extraction (LLE) followed by liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS) recorded in positive ion mode. Saliva samples were collected by spitting directly into tubes and 250 µL were used for analysis. The limits of detection were 4.1 pmol/L, 0.27 nmol/L and 10.8 pmol/L for melatonin, cortisol, and testosterone, respectively. The developed method was sensitive enough to measure circadian rhythms of all 3 hormones in a pilot study among four healthy volunteers. It can therefor be used to study the impact of night work and working in artificial light on the workers circadian rhythms. To our knowledge this is the first LC-ESI-MS/MS method for simultaneous determination of salivary melatonin, cortisol and testosterone.
AB - Circadian disruption can have several possible health consequences, but is not well studied. In order to measure circadian disruption, in relation to shift or night work, we developed a simple and sensitive method for the simultaneous determination of melatonin, cortisol and testosterone in human saliva. We used liquid-liquid extraction (LLE) followed by liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS) recorded in positive ion mode. Saliva samples were collected by spitting directly into tubes and 250 µL were used for analysis. The limits of detection were 4.1 pmol/L, 0.27 nmol/L and 10.8 pmol/L for melatonin, cortisol, and testosterone, respectively. The developed method was sensitive enough to measure circadian rhythms of all 3 hormones in a pilot study among four healthy volunteers. It can therefor be used to study the impact of night work and working in artificial light on the workers circadian rhythms. To our knowledge this is the first LC-ESI-MS/MS method for simultaneous determination of salivary melatonin, cortisol and testosterone.
KW - Adult
KW - Chromatography, Liquid
KW - Circadian Rhythm
KW - Drug Stability
KW - Female
KW - Humans
KW - Hydrocortisone
KW - Male
KW - Melatonin
KW - Middle Aged
KW - Reproducibility of Results
KW - Saliva
KW - Tandem Mass Spectrometry
KW - Testosterone
U2 - 10.1016/j.jchromb.2011.07.005
DO - 10.1016/j.jchromb.2011.07.005
M3 - Journal article
C2 - 21803007
VL - 879
SP - 2527
EP - 2532
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 25
ER -
ID: 37472858