Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy. / Hägglund, P.; Sabini, E.; Boisset, C.; Wilson, K.; Chanzy, H.; Stålbrand, H.

In: Biomacromolecules, Vol. 2, No. 3, 04.10.2001, p. 694-699.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hägglund, P, Sabini, E, Boisset, C, Wilson, K, Chanzy, H & Stålbrand, H 2001, 'Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy', Biomacromolecules, vol. 2, no. 3, pp. 694-699. https://doi.org/10.1021/bm010006w

APA

Hägglund, P., Sabini, E., Boisset, C., Wilson, K., Chanzy, H., & Stålbrand, H. (2001). Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy. Biomacromolecules, 2(3), 694-699. https://doi.org/10.1021/bm010006w

Vancouver

Hägglund P, Sabini E, Boisset C, Wilson K, Chanzy H, Stålbrand H. Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy. Biomacromolecules. 2001 Oct 4;2(3):694-699. https://doi.org/10.1021/bm010006w

Author

Hägglund, P. ; Sabini, E. ; Boisset, C. ; Wilson, K. ; Chanzy, H. ; Stålbrand, H. / Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy. In: Biomacromolecules. 2001 ; Vol. 2, No. 3. pp. 694-699.

Bibtex

@article{b11658a2d2d348b1934406eb63f77ada,
title = "Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy",
abstract = "We have used the endo-β-1,4-mannanase from Trichoderma reesei (Tr Man5A), the endo-β-1,4-mannanase from Aspergillus niger (An Man5A) and the exo-β-1,4-mannosidase from A. niger (An Mnd2A) to follow the enzymatic degradation of mannan I and II crystals. The degradation process was studied by transmission electron microscopy and also followed by analysis of the released soluble reducing sugars. The mannan crystals were degraded by the endo-β-1,4-mannanases and to a lesser extent by the exo-β-1,4-mannosidase. The observed hydrolysis pattern on mannan I crystals is fully consistent with the current view of the molecular structure of these crystals. The molecular organization of the mannan chains in mannan II crystals is less clear and the digestion results give some further information about the ultrastructure of mannan II. In addition, insight is provided into the mode of the enzymatic attack on the crystals of mannan I and mannan II.",
author = "P. H{\"a}gglund and E. Sabini and C. Boisset and K. Wilson and H. Chanzy and H. St{\aa}lbrand",
year = "2001",
month = oct,
day = "4",
doi = "10.1021/bm010006w",
language = "English",
volume = "2",
pages = "694--699",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "3",

}

RIS

TY - JOUR

T1 - Degradation of mannan I and II crystals by fungal endo-β-1,4-mannanases and a β-1,4-mannosidase studied with transmission electron microscopy

AU - Hägglund, P.

AU - Sabini, E.

AU - Boisset, C.

AU - Wilson, K.

AU - Chanzy, H.

AU - Stålbrand, H.

PY - 2001/10/4

Y1 - 2001/10/4

N2 - We have used the endo-β-1,4-mannanase from Trichoderma reesei (Tr Man5A), the endo-β-1,4-mannanase from Aspergillus niger (An Man5A) and the exo-β-1,4-mannosidase from A. niger (An Mnd2A) to follow the enzymatic degradation of mannan I and II crystals. The degradation process was studied by transmission electron microscopy and also followed by analysis of the released soluble reducing sugars. The mannan crystals were degraded by the endo-β-1,4-mannanases and to a lesser extent by the exo-β-1,4-mannosidase. The observed hydrolysis pattern on mannan I crystals is fully consistent with the current view of the molecular structure of these crystals. The molecular organization of the mannan chains in mannan II crystals is less clear and the digestion results give some further information about the ultrastructure of mannan II. In addition, insight is provided into the mode of the enzymatic attack on the crystals of mannan I and mannan II.

AB - We have used the endo-β-1,4-mannanase from Trichoderma reesei (Tr Man5A), the endo-β-1,4-mannanase from Aspergillus niger (An Man5A) and the exo-β-1,4-mannosidase from A. niger (An Mnd2A) to follow the enzymatic degradation of mannan I and II crystals. The degradation process was studied by transmission electron microscopy and also followed by analysis of the released soluble reducing sugars. The mannan crystals were degraded by the endo-β-1,4-mannanases and to a lesser extent by the exo-β-1,4-mannosidase. The observed hydrolysis pattern on mannan I crystals is fully consistent with the current view of the molecular structure of these crystals. The molecular organization of the mannan chains in mannan II crystals is less clear and the digestion results give some further information about the ultrastructure of mannan II. In addition, insight is provided into the mode of the enzymatic attack on the crystals of mannan I and mannan II.

UR - http://www.scopus.com/inward/record.url?scp=0034831908&partnerID=8YFLogxK

U2 - 10.1021/bm010006w

DO - 10.1021/bm010006w

M3 - Journal article

C2 - 11710023

AN - SCOPUS:0034831908

VL - 2

SP - 694

EP - 699

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 3

ER -

ID: 240162217