Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene
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An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml-1 to 16 µg ml-1, which represents a significant improvement of the original version.
Original language | English |
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Journal | FEMS Microbiology Letters |
Volume | 253 |
Issue number | 2 |
Pages (from-to) | 201-205 |
ISSN | 0378-1097 |
DOIs | |
Publication status | Published - 2005 |
Bibliographical note
Keywords: Biosensor; Flow cytometry; Tetracycline
ID: 87541