Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis. / Nielsen, Sara Elsøe; Vainer, Ben; Nielsen, Ole Haagen.

In: Digestive Diseases and Sciences, Vol. 49, No. 5, 01.05.2004, p. 878-882.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, SE, Vainer, B & Nielsen, OH 2004, 'Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis', Digestive Diseases and Sciences, vol. 49, no. 5, pp. 878-882. https://doi.org/10.1023/B:DDAS.0000030103.85580.d6

APA

Nielsen, S. E., Vainer, B., & Nielsen, O. H. (2004). Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis. Digestive Diseases and Sciences, 49(5), 878-882. https://doi.org/10.1023/B:DDAS.0000030103.85580.d6

Vancouver

Nielsen SE, Vainer B, Nielsen OH. Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis. Digestive Diseases and Sciences. 2004 May 1;49(5):878-882. https://doi.org/10.1023/B:DDAS.0000030103.85580.d6

Author

Nielsen, Sara Elsøe ; Vainer, Ben ; Nielsen, Ole Haagen. / Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis. In: Digestive Diseases and Sciences. 2004 ; Vol. 49, No. 5. pp. 878-882.

Bibtex

@article{8b66e733316240d6abfab35015f3eebc,
title = "Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis",
abstract = "A diminished tolerance to the normal gut bacterial flora has been suggested to be pathogenic in ulcerative colitis (UC) and the aim of this study was to evaluate the priming effect of selected bacterial wall products on UC neutrophil granulocytes. Neutrophils from 10 UC patients and 10 healthy controls were primed with bacterial lipoprotein (BLP) or lipopolysaccharide (LPS) and subsequently activated. Extracellular superoxide production was measured by the cytochrome c reduction assay. Priming neutrophils with BLP or LPS dose dependently increased the superoxide production in both UC and controls (P<0.01), and BLP was more potent than LPS (P<0.05). No differences were found between UC and controls. UC neutrophils do not seem to have an intrinsic abnormality with reduced tolerance to bacterial substances. However, bacterial wall products such as BLP modify neutrophil tissue-destruction mechanisms and might be pivotal for perpetuation of chronic colonic inflammation.",
keywords = "bacterial lipoprotein, cytochrome c, neutrophils, superoxide, ulcerative colitis",
author = "Nielsen, {Sara Els{\o}e} and Ben Vainer and Nielsen, {Ole Haagen}",
year = "2004",
month = may,
day = "1",
doi = "10.1023/B:DDAS.0000030103.85580.d6",
language = "English",
volume = "49",
pages = "878--882",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - Comparative studies of superoxide production by microbial wall product-primed neutrophils in ulcerative colitis

AU - Nielsen, Sara Elsøe

AU - Vainer, Ben

AU - Nielsen, Ole Haagen

PY - 2004/5/1

Y1 - 2004/5/1

N2 - A diminished tolerance to the normal gut bacterial flora has been suggested to be pathogenic in ulcerative colitis (UC) and the aim of this study was to evaluate the priming effect of selected bacterial wall products on UC neutrophil granulocytes. Neutrophils from 10 UC patients and 10 healthy controls were primed with bacterial lipoprotein (BLP) or lipopolysaccharide (LPS) and subsequently activated. Extracellular superoxide production was measured by the cytochrome c reduction assay. Priming neutrophils with BLP or LPS dose dependently increased the superoxide production in both UC and controls (P<0.01), and BLP was more potent than LPS (P<0.05). No differences were found between UC and controls. UC neutrophils do not seem to have an intrinsic abnormality with reduced tolerance to bacterial substances. However, bacterial wall products such as BLP modify neutrophil tissue-destruction mechanisms and might be pivotal for perpetuation of chronic colonic inflammation.

AB - A diminished tolerance to the normal gut bacterial flora has been suggested to be pathogenic in ulcerative colitis (UC) and the aim of this study was to evaluate the priming effect of selected bacterial wall products on UC neutrophil granulocytes. Neutrophils from 10 UC patients and 10 healthy controls were primed with bacterial lipoprotein (BLP) or lipopolysaccharide (LPS) and subsequently activated. Extracellular superoxide production was measured by the cytochrome c reduction assay. Priming neutrophils with BLP or LPS dose dependently increased the superoxide production in both UC and controls (P<0.01), and BLP was more potent than LPS (P<0.05). No differences were found between UC and controls. UC neutrophils do not seem to have an intrinsic abnormality with reduced tolerance to bacterial substances. However, bacterial wall products such as BLP modify neutrophil tissue-destruction mechanisms and might be pivotal for perpetuation of chronic colonic inflammation.

KW - bacterial lipoprotein

KW - cytochrome c

KW - neutrophils

KW - superoxide

KW - ulcerative colitis

UR - http://www.scopus.com/inward/record.url?scp=3042764298&partnerID=8YFLogxK

U2 - 10.1023/B:DDAS.0000030103.85580.d6

DO - 10.1023/B:DDAS.0000030103.85580.d6

M3 - Journal article

C2 - 15259513

AN - SCOPUS:3042764298

VL - 49

SP - 878

EP - 882

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 5

ER -

ID: 218708469