Collection of apoplastic fluids from Arabidopsis thaliana leaves
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.
Original language | English |
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Title of host publication | Biotechnology of plant secondary metabolism : methods and protocols |
Number of pages | 8 |
Publisher | Springer |
Publication date | 2016 |
Pages | 35-42 |
Chapter | 4 |
ISBN (Print) | 978-1-4939-3391-4 |
ISBN (Electronic) | 978-1-4939-3393-8 |
DOIs | |
Publication status | Published - 2016 |
Series | Methods in Molecular Biology |
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Volume | 1405 |
ISSN | 1064-3745 |
ID: 161274527