Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics
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Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics. / Yucel Falco, Cigdem; Falkman, Peter; Risbo, Jens; Cárdenas, Marité; Medronho, Bruno.
In: Carbohydrate Polymers, Vol. 172, 2017, p. 175-183.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics
AU - Yucel Falco, Cigdem
AU - Falkman, Peter
AU - Risbo, Jens
AU - Cárdenas, Marité
AU - Medronho, Bruno
PY - 2017
Y1 - 2017
N2 - Physical and chemical (crosslinked with genipin) hydrogels based on chitosan and dextran sulfate were developed and characterized as novel bio-materials suitable for probiotic encapsulation. The swelling of the hydrogels was dependent on the composition and weakly influenced by the pH of the media. The morphology analysis supports the swelling data showing distinct changes in microstructure depending on the composition. The viability and culturability tests showed approx. 3.6 log CFU/mL decrease of cells (L. acidophilus as model) incorporated into chemical hydrogels when compared to the number of viable native cells. However, the live/dead viability assay evidenced that a considerable amount of viable cells were still entrapped in the hydrogel network and therefore the viability is most likely underestimated. Overall, the developed systems are robust and their structure, rheology and swelling properties can be tuned by changing the blend ratio, thus constituting appealing bio-matrices for cell encapsulation.
AB - Physical and chemical (crosslinked with genipin) hydrogels based on chitosan and dextran sulfate were developed and characterized as novel bio-materials suitable for probiotic encapsulation. The swelling of the hydrogels was dependent on the composition and weakly influenced by the pH of the media. The morphology analysis supports the swelling data showing distinct changes in microstructure depending on the composition. The viability and culturability tests showed approx. 3.6 log CFU/mL decrease of cells (L. acidophilus as model) incorporated into chemical hydrogels when compared to the number of viable native cells. However, the live/dead viability assay evidenced that a considerable amount of viable cells were still entrapped in the hydrogel network and therefore the viability is most likely underestimated. Overall, the developed systems are robust and their structure, rheology and swelling properties can be tuned by changing the blend ratio, thus constituting appealing bio-matrices for cell encapsulation.
KW - Chitosan
KW - Dextran sulfate
KW - Encapsulation
KW - Genipin
KW - Hydrogel
KW - Probiotic bacteria
U2 - 10.1016/j.carbpol.2017.04.047
DO - 10.1016/j.carbpol.2017.04.047
M3 - Journal article
C2 - 28606523
AN - SCOPUS:85019562805
VL - 172
SP - 175
EP - 183
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
ER -
ID: 179122029