Breeding of Hibiscus rosa-sinensis for garden use in Denmark
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Breeding of Hibiscus rosa-sinensis for garden use in Denmark. / Kuligowska, Katarzyna; Simonsen, Maria Boye; Lütken, Henrik Vlk; Müller, Renate; Christensen, B.
In: Acta Horticulturae, Vol. 990, 2013, p. 235-241.Research output: Contribution to journal › Conference article › Research › peer-review
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TY - GEN
T1 - Breeding of Hibiscus rosa-sinensis for garden use in Denmark
AU - Kuligowska, Katarzyna
AU - Simonsen, Maria Boye
AU - Lütken, Henrik Vlk
AU - Müller, Renate
AU - Christensen, B.
N1 - Conference code: 11
PY - 2013
Y1 - 2013
N2 - Hibiscus rosa-sinensis is an ornamental shrub in tropical and subtropical regions. Limitation of its use as an outdoor ornamental plant in a temperate climate is due to chilling sensitivity. Cold hardiness is a highly complex trait, involving multiple genes. Improvement of H. rosa-sinensis for increased chilling tolerance by conventional breeding is unlikely because there is a lack of genetic information in relation to this trait within this species. In the Hibiscus genus, cold hardiness is available, but only in a few species. Attempts to create cold tolerant Hibiscus plants resembling H. rosa-sinensis suitable for Nordic climate have not been successful so far. In ornamental breeding wide hybridization is a major source of genetic variation, and can also be a promising approach to introgress cold hardiness in H. rosa-sinensis. The objective of the present study is to evaluate the possibilities of interspecific hybridization between H. rosa-sinensis and cold tolerant Hibiscus species. The main focus was to identify prefertilization hybridization barriers using microscopic methods. Pollen viability was determined by staining with acetocarmine. An attempt to resolve the ability of pollen grains to germinate was made in vitro using artificial medium. In situ pollen germination and pollen tube growth were monitored using staining with aniline blue. Crosses between H. rosa-sinensis and H. syriacus or H. moscheutos, did not result in seed formation, due to hybridization barriers.
AB - Hibiscus rosa-sinensis is an ornamental shrub in tropical and subtropical regions. Limitation of its use as an outdoor ornamental plant in a temperate climate is due to chilling sensitivity. Cold hardiness is a highly complex trait, involving multiple genes. Improvement of H. rosa-sinensis for increased chilling tolerance by conventional breeding is unlikely because there is a lack of genetic information in relation to this trait within this species. In the Hibiscus genus, cold hardiness is available, but only in a few species. Attempts to create cold tolerant Hibiscus plants resembling H. rosa-sinensis suitable for Nordic climate have not been successful so far. In ornamental breeding wide hybridization is a major source of genetic variation, and can also be a promising approach to introgress cold hardiness in H. rosa-sinensis. The objective of the present study is to evaluate the possibilities of interspecific hybridization between H. rosa-sinensis and cold tolerant Hibiscus species. The main focus was to identify prefertilization hybridization barriers using microscopic methods. Pollen viability was determined by staining with acetocarmine. An attempt to resolve the ability of pollen grains to germinate was made in vitro using artificial medium. In situ pollen germination and pollen tube growth were monitored using staining with aniline blue. Crosses between H. rosa-sinensis and H. syriacus or H. moscheutos, did not result in seed formation, due to hybridization barriers.
M3 - Conference article
VL - 990
SP - 235
EP - 241
JO - Acta Horticulturae
JF - Acta Horticulturae
SN - 0567-7572
Y2 - 1 July 2012 through 4 July 2012
ER -
ID: 95073234