Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy

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Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy. / Andersen, Trine Bundgaard; Rasmussen, Silas Anselm; Christensen, Søren Brøgger; Simonsen, Henrik Toft.

In: Phytochemistry, Vol. 157, No. https://doi.org/10.1016/j.phytochem.2018.10.027, 01.2019, p. 168-174.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andersen, TB, Rasmussen, SA, Christensen, SB & Simonsen, HT 2019, 'Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy', Phytochemistry, vol. 157, no. https://doi.org/10.1016/j.phytochem.2018.10.027, pp. 168-174. https://doi.org/10.1016/j.phytochem.2018.10.027

APA

Andersen, T. B., Rasmussen, S. A., Christensen, S. B., & Simonsen, H. T. (2019). Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy. Phytochemistry, 157(https://doi.org/10.1016/j.phytochem.2018.10.027), 168-174. https://doi.org/10.1016/j.phytochem.2018.10.027

Vancouver

Andersen TB, Rasmussen SA, Christensen SB, Simonsen HT. Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy. Phytochemistry. 2019 Jan;157(https://doi.org/10.1016/j.phytochem.2018.10.027):168-174. https://doi.org/10.1016/j.phytochem.2018.10.027

Author

Andersen, Trine Bundgaard ; Rasmussen, Silas Anselm ; Christensen, Søren Brøgger ; Simonsen, Henrik Toft. / Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy. In: Phytochemistry. 2019 ; Vol. 157, No. https://doi.org/10.1016/j.phytochem.2018.10.027. pp. 168-174.

Bibtex

@article{7bd9dff6d80f4e378f3181be3dadd922,
title = "Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy",
abstract = "The genus Thapsia produces a wide variety of sesquiterpenoids. The Mediterranean plant Thapsia laciniata Rouyis known to have a product profile that differs from several other species in the genus. Thus, the biosynthesis ofsesquiterpenoids in Thapsia laciniata Rouy was investigated. Here we describe three terpene synthases,TlTPS820, TlTPS509 and TlTPS18983. TlTPS18983 is a multi-product enzyme with farnesene as the majorproduct, while TlTPS509 produces guaiol and bulnesol along with other major and several minor unknownproducts. TlTPS820 is orthologous to TgTPS2 from Thapsia garganica L. and is an epikunzeaol synthase.TgCYP76AE2 from Thapsia garganica performs a triple hydroxylation of epikunzeaol at C-12 to make dihydrocostunolide.It was therefore investigated if the cytochrome P450, TlCYP76AE4 was able to use epikunzeaolas a substrate. It was found that TlCYP76AE4 hydroxylates epikunzeaol at C-8 to yield tovarol instead of dihydrocostunolide.",
author = "Andersen, {Trine Bundgaard} and Rasmussen, {Silas Anselm} and Christensen, {S{\o}ren Br{\o}gger} and Simonsen, {Henrik Toft}",
year = "2019",
month = jan,
doi = "10.1016/j.phytochem.2018.10.027",
language = "English",
volume = "157",
pages = "168--174",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Pergamon Press",
number = "https://doi.org/10.1016/j.phytochem.2018.10.027",

}

RIS

TY - JOUR

T1 - Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy

AU - Andersen, Trine Bundgaard

AU - Rasmussen, Silas Anselm

AU - Christensen, Søren Brøgger

AU - Simonsen, Henrik Toft

PY - 2019/1

Y1 - 2019/1

N2 - The genus Thapsia produces a wide variety of sesquiterpenoids. The Mediterranean plant Thapsia laciniata Rouyis known to have a product profile that differs from several other species in the genus. Thus, the biosynthesis ofsesquiterpenoids in Thapsia laciniata Rouy was investigated. Here we describe three terpene synthases,TlTPS820, TlTPS509 and TlTPS18983. TlTPS18983 is a multi-product enzyme with farnesene as the majorproduct, while TlTPS509 produces guaiol and bulnesol along with other major and several minor unknownproducts. TlTPS820 is orthologous to TgTPS2 from Thapsia garganica L. and is an epikunzeaol synthase.TgCYP76AE2 from Thapsia garganica performs a triple hydroxylation of epikunzeaol at C-12 to make dihydrocostunolide.It was therefore investigated if the cytochrome P450, TlCYP76AE4 was able to use epikunzeaolas a substrate. It was found that TlCYP76AE4 hydroxylates epikunzeaol at C-8 to yield tovarol instead of dihydrocostunolide.

AB - The genus Thapsia produces a wide variety of sesquiterpenoids. The Mediterranean plant Thapsia laciniata Rouyis known to have a product profile that differs from several other species in the genus. Thus, the biosynthesis ofsesquiterpenoids in Thapsia laciniata Rouy was investigated. Here we describe three terpene synthases,TlTPS820, TlTPS509 and TlTPS18983. TlTPS18983 is a multi-product enzyme with farnesene as the majorproduct, while TlTPS509 produces guaiol and bulnesol along with other major and several minor unknownproducts. TlTPS820 is orthologous to TgTPS2 from Thapsia garganica L. and is an epikunzeaol synthase.TgCYP76AE2 from Thapsia garganica performs a triple hydroxylation of epikunzeaol at C-12 to make dihydrocostunolide.It was therefore investigated if the cytochrome P450, TlCYP76AE4 was able to use epikunzeaolas a substrate. It was found that TlCYP76AE4 hydroxylates epikunzeaol at C-8 to yield tovarol instead of dihydrocostunolide.

U2 - 10.1016/j.phytochem.2018.10.027

DO - 10.1016/j.phytochem.2018.10.027

M3 - Journal article

C2 - 30412824

VL - 157

SP - 168

EP - 174

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - https://doi.org/10.1016/j.phytochem.2018.10.027

ER -

ID: 204530534