Bioanalysis of drugs by liquid-phase microextraction coupled to separation techniques
Research output: Contribution to journal › Review › Research › peer-review
The demand for automation of liquid-liquid extraction (LLE) in drug analysis combined with the demand for reduced sample preparation time has led to the recent development of liquid-phase microextraction (LPME) based on disposable hollow fibres. In LPME, target drugs are extracted from aqueous biological samples, through a thin layer of organic solvent immobilised within the pores of the wall of a porous hollow fibre, and into an μl volume of acceptor solution inside the lumen of the hollow fibre. After extraction, the acceptor solution is subjected directly to a final analysis either by high performance liquid chromatography (HPLC), capillary electrophoresis (CE), mass spectrometry (MS), or capillary gas chromatography (GC) without any further treatments. Hollow fibre-based LPME may provide high enrichment of drugs and excellent sample clean-up, and probably has a broad application potential within the area of drug analysis. This review focuses on the principle of LPME, and recent applications of three-phase, two-phase, and carrier mediated LPME of drugs from plasma, whole blood, urine, and breast milk.
Original language | English |
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Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
Volume | 817 |
Issue number | 1 |
Pages (from-to) | 3-12 |
Number of pages | 10 |
ISSN | 1570-0232 |
DOIs | |
Publication status | Published - 5 Mar 2005 |
- Breast milk, Drug analysis, Hollow fibres, Liquid-phase microextraction, Plasma, Urine, Whole blood
Research areas
ID: 231651971