Bacterial community development and diversity during the first year of production in a new salmon processing plant
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Bacterial community development and diversity during the first year of production in a new salmon processing plant. / Thomassen, Gunn Merethe Bjørge; Krych, Lukasz; Knøchel, Susanne; Mehli, Lisbeth.
In: Food Microbiology, Vol. 109, 104138, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Bacterial community development and diversity during the first year of production in a new salmon processing plant
AU - Thomassen, Gunn Merethe Bjørge
AU - Krych, Lukasz
AU - Knøchel, Susanne
AU - Mehli, Lisbeth
N1 - Publisher Copyright: © 2022 The Author(s)
PY - 2023
Y1 - 2023
N2 - The bacterial diversity and load on equipment in food processing facilities is constantly influenced by raw material, water, air, and staff. Despite regular cleaning and disinfection, some bacteria may persist and thereby potentially compromise food quality and safety. Little is known about how bacterial communities in a new food processing facility gradually establish themselves. Here, the development of bacterial communities in a newly opened salmon processing plant was studied from the first day and during the first year of operation. To focus on the persisting bacterial communities, surface sampling was done on strategical sampling points after cleaning and disinfection. To study the diversity dynamics, isolates from selected sampling and time points were classified by Oxford Nanopore Technology-based rep-PCR amplicon sequencing (ON-rep-seq) supplemented by 16S rRNA gene or rpoD gene sequencing (for Pseudomonas). An overall increase in bacterial numbers was only observed for food-contact surfaces in the slaughter department, but not in filleting department, on non-food contact surfaces or on the fish. Changes in temporal and spatial diversity and community composition were observed and our approach revealed highly point-specific bacterial communities.
AB - The bacterial diversity and load on equipment in food processing facilities is constantly influenced by raw material, water, air, and staff. Despite regular cleaning and disinfection, some bacteria may persist and thereby potentially compromise food quality and safety. Little is known about how bacterial communities in a new food processing facility gradually establish themselves. Here, the development of bacterial communities in a newly opened salmon processing plant was studied from the first day and during the first year of operation. To focus on the persisting bacterial communities, surface sampling was done on strategical sampling points after cleaning and disinfection. To study the diversity dynamics, isolates from selected sampling and time points were classified by Oxford Nanopore Technology-based rep-PCR amplicon sequencing (ON-rep-seq) supplemented by 16S rRNA gene or rpoD gene sequencing (for Pseudomonas). An overall increase in bacterial numbers was only observed for food-contact surfaces in the slaughter department, but not in filleting department, on non-food contact surfaces or on the fish. Changes in temporal and spatial diversity and community composition were observed and our approach revealed highly point-specific bacterial communities.
KW - Bacterial community
KW - Food processing environment
KW - Food safety
KW - Food spoilage
KW - ON-Rep-seq
KW - Species-level identification
U2 - 10.1016/j.fm.2022.104138
DO - 10.1016/j.fm.2022.104138
M3 - Journal article
C2 - 36309440
AN - SCOPUS:85139243758
VL - 109
JO - Food Microbiology
JF - Food Microbiology
SN - 0740-0020
M1 - 104138
ER -
ID: 324965506