Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique
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Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique. / Thymann, M; Nellemann, L J; Masumba, G; Irgens-Møller, L; Morling, N.
In: Forensic Science International, Vol. 60, No. 1-2, 1993, p. 47-56.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique
AU - Thymann, M
AU - Nellemann, L J
AU - Masumba, G
AU - Irgens-Møller, L
AU - Morling, N
N1 - Keywords: Adult; Alleles; Child; DNA; Denmark; European Continental Ancestry Group; Female; Humans; Male; Mothers; Paternity; Phenotype; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Reproducibility of Results
PY - 1993
Y1 - 1993
N2 - DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) analysis of the D1S80 locus demonstrated 21 alleles and a heterozygosity of 77%. Of the 231 possible phenotypes, 57 were observed. All mother/child pairs shared at least one D1S80 allele. The D1S80 typing results in 70 Danes were compared to the results obtained on the same samples in another laboratory and the results were concordant in all cases.
AB - DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) analysis of the D1S80 locus demonstrated 21 alleles and a heterozygosity of 77%. Of the 231 possible phenotypes, 57 were observed. All mother/child pairs shared at least one D1S80 allele. The D1S80 typing results in 70 Danes were compared to the results obtained on the same samples in another laboratory and the results were concordant in all cases.
M3 - Journal article
C2 - 8101829
VL - 60
SP - 47
EP - 56
JO - Forensic Science International
JF - Forensic Science International
SN - 0379-0738
IS - 1-2
ER -
ID: 16186204