An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium. / Ekelund, Flemming; Christensen, Søren; Rønn, Regin; Buhl, E.; Jacobsen, C.S.

In: Journal of Microbiological Methods, Vol. 38, No. 3, 1999, p. 177-182.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ekelund, F, Christensen, S, Rønn, R, Buhl, E & Jacobsen, CS 1999, 'An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium', Journal of Microbiological Methods, vol. 38, no. 3, pp. 177-182. https://doi.org/10.1016/S0167-7012(99)00086-X

APA

Ekelund, F., Christensen, S., Rønn, R., Buhl, E., & Jacobsen, C. S. (1999). An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium. Journal of Microbiological Methods, 38(3), 177-182. https://doi.org/10.1016/S0167-7012(99)00086-X

Vancouver

Ekelund F, Christensen S, Rønn R, Buhl E, Jacobsen CS. An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium. Journal of Microbiological Methods. 1999;38(3):177-182. https://doi.org/10.1016/S0167-7012(99)00086-X

Author

Ekelund, Flemming ; Christensen, Søren ; Rønn, Regin ; Buhl, E. ; Jacobsen, C.S. / An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium. In: Journal of Microbiological Methods. 1999 ; Vol. 38, No. 3. pp. 177-182.

Bibtex

@article{a945c0403d5811ddb7b4000ea68e967b,

title = "An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium",

abstract = "An automated modification of the most-probable-number (MPN) technique has been developed for enumeration of phagotrophic protozoa. The method is based on detection of prey depletion in micro titre plates rather than on presence of protozoa. A transconjugant Pseudomonas fluorescens DR54 labelled with a luxAB gene cassette was constructed, and used as growth medium for the protozoa in the micro titre plates. The transconjugant produced high amounts of luciferase which was stable and allowed detection for at least 8 weeks. Dilution series of protozoan cultures and soil suspensions were inoculated into micro titre plates amended with a suspension of the transconjugant. After 45 days measurement of light emission allowed detection of individual wells in the titre plates, where protozoan grazing had removed the inoculated bacteria.",

author = "Flemming Ekelund and S{\o}ren Christensen and Regin R{\o}nn and E. Buhl and C.S. Jacobsen",

note = "Author Keywords: Heterotrophic flagellates; Lux AB labelled bacteria; Naked amoebae; Protozoa; Sediment; Soil",

year = "1999",

doi = "10.1016/S0167-7012(99)00086-X",

language = "English",

volume = "38",

pages = "177--182",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "3",

}

RIS

TY - JOUR

T1 - An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux-AB labelled bacteria as growth medium

AU - Ekelund, Flemming

AU - Christensen, Søren

AU - Rønn, Regin

AU - Buhl, E.

AU - Jacobsen, C.S.

N1 - Author Keywords: Heterotrophic flagellates; Lux AB labelled bacteria; Naked amoebae; Protozoa; Sediment; Soil

PY - 1999

Y1 - 1999

N2 - An automated modification of the most-probable-number (MPN) technique has been developed for enumeration of phagotrophic protozoa. The method is based on detection of prey depletion in micro titre plates rather than on presence of protozoa. A transconjugant Pseudomonas fluorescens DR54 labelled with a luxAB gene cassette was constructed, and used as growth medium for the protozoa in the micro titre plates. The transconjugant produced high amounts of luciferase which was stable and allowed detection for at least 8 weeks. Dilution series of protozoan cultures and soil suspensions were inoculated into micro titre plates amended with a suspension of the transconjugant. After 45 days measurement of light emission allowed detection of individual wells in the titre plates, where protozoan grazing had removed the inoculated bacteria.

AB - An automated modification of the most-probable-number (MPN) technique has been developed for enumeration of phagotrophic protozoa. The method is based on detection of prey depletion in micro titre plates rather than on presence of protozoa. A transconjugant Pseudomonas fluorescens DR54 labelled with a luxAB gene cassette was constructed, and used as growth medium for the protozoa in the micro titre plates. The transconjugant produced high amounts of luciferase which was stable and allowed detection for at least 8 weeks. Dilution series of protozoan cultures and soil suspensions were inoculated into micro titre plates amended with a suspension of the transconjugant. After 45 days measurement of light emission allowed detection of individual wells in the titre plates, where protozoan grazing had removed the inoculated bacteria.

U2 - 10.1016/S0167-7012(99)00086-X

DO - 10.1016/S0167-7012(99)00086-X

M3 - Journal article

VL - 38

SP - 177

EP - 182

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 3

ER -

ID: 4625146