Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA

Research output: Contribution to journalJournal articlepeer-review

Standard

Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA. / Mikkelsen, Lone; Bialkowski, Karol; Risom, Lotte; Løhr, Mille; Loft, Steffen; Møller, Peter.

In: Free Radical Biology & Medicine, Vol. 47, No. 5, 2009, p. 608-15.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Mikkelsen, L, Bialkowski, K, Risom, L, Løhr, M, Loft, S & Møller, P 2009, 'Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA', Free Radical Biology & Medicine, vol. 47, no. 5, pp. 608-15. https://doi.org/10.1016/j.freeradbiomed.2009.05.030

APA

Mikkelsen, L., Bialkowski, K., Risom, L., Løhr, M., Loft, S., & Møller, P. (2009). Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA. Free Radical Biology & Medicine, 47(5), 608-15. https://doi.org/10.1016/j.freeradbiomed.2009.05.030

Vancouver

Mikkelsen L, Bialkowski K, Risom L, Løhr M, Loft S, Møller P. Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA. Free Radical Biology & Medicine. 2009;47(5):608-15. https://doi.org/10.1016/j.freeradbiomed.2009.05.030

Author

Mikkelsen, Lone ; Bialkowski, Karol ; Risom, Lotte ; Løhr, Mille ; Loft, Steffen ; Møller, Peter. / Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA. In: Free Radical Biology & Medicine. 2009 ; Vol. 47, No. 5. pp. 608-15.

Bibtex

@article{975bc6a0a10b11debc73000ea68e967b,
title = "Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA",
abstract = "The imbalance between the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in DNA and the efficiency of cellular systems of DNA protection and repair is considered an important factor in the age-dependent development of cancer. This study investigated the relationship between oxidatively damaged DNA and the activity of the DNA repair system and 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate pyrophosphohydrolase (8-oxodGTPase) activity in liver and lung tissue from mice at 10-100 weeks of age. The level of 8-oxodG increased with age, whereas the level of formamidopyrimidine DNA glycosylase sites was unaltered. The enzyme activity toward single oxygen-induced DNA damage and mRNA expression levels of Ercc1, Neil1, and Ogg1 remained unaltered with age. However, the 8-oxodGTPase activity in the liver was 18% (95% CI: 0.2-37%) lower in mice at 25 and 50 weeks than in 10-week-old mice. The 10- and 100-week-old mice had similar 8-oxodGTPase activity. In contrast, the mRNA expression of Nudt1 was statistically unaltered that likely resulted from higher variation of measurements. The accumulation of 8-oxodG with age is not a direct consequence of decreased enzyme activity toward singlet oxygen-induced substrate DNA. An age-related higher level of 8-oxodG even occurs concomitantly with high 8-oxodGTPase activity.",
author = "Lone Mikkelsen and Karol Bialkowski and Lotte Risom and Mille L{\o}hr and Steffen Loft and Peter M{\o}ller",
year = "2009",
doi = "10.1016/j.freeradbiomed.2009.05.030",
language = "English",
volume = "47",
pages = "608--15",
journal = "Free Radical Biology & Medicine",
issn = "0891-5849",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Aging and defense against generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA

AU - Mikkelsen, Lone

AU - Bialkowski, Karol

AU - Risom, Lotte

AU - Løhr, Mille

AU - Loft, Steffen

AU - Møller, Peter

PY - 2009

Y1 - 2009

N2 - The imbalance between the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in DNA and the efficiency of cellular systems of DNA protection and repair is considered an important factor in the age-dependent development of cancer. This study investigated the relationship between oxidatively damaged DNA and the activity of the DNA repair system and 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate pyrophosphohydrolase (8-oxodGTPase) activity in liver and lung tissue from mice at 10-100 weeks of age. The level of 8-oxodG increased with age, whereas the level of formamidopyrimidine DNA glycosylase sites was unaltered. The enzyme activity toward single oxygen-induced DNA damage and mRNA expression levels of Ercc1, Neil1, and Ogg1 remained unaltered with age. However, the 8-oxodGTPase activity in the liver was 18% (95% CI: 0.2-37%) lower in mice at 25 and 50 weeks than in 10-week-old mice. The 10- and 100-week-old mice had similar 8-oxodGTPase activity. In contrast, the mRNA expression of Nudt1 was statistically unaltered that likely resulted from higher variation of measurements. The accumulation of 8-oxodG with age is not a direct consequence of decreased enzyme activity toward singlet oxygen-induced substrate DNA. An age-related higher level of 8-oxodG even occurs concomitantly with high 8-oxodGTPase activity.

AB - The imbalance between the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in DNA and the efficiency of cellular systems of DNA protection and repair is considered an important factor in the age-dependent development of cancer. This study investigated the relationship between oxidatively damaged DNA and the activity of the DNA repair system and 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate pyrophosphohydrolase (8-oxodGTPase) activity in liver and lung tissue from mice at 10-100 weeks of age. The level of 8-oxodG increased with age, whereas the level of formamidopyrimidine DNA glycosylase sites was unaltered. The enzyme activity toward single oxygen-induced DNA damage and mRNA expression levels of Ercc1, Neil1, and Ogg1 remained unaltered with age. However, the 8-oxodGTPase activity in the liver was 18% (95% CI: 0.2-37%) lower in mice at 25 and 50 weeks than in 10-week-old mice. The 10- and 100-week-old mice had similar 8-oxodGTPase activity. In contrast, the mRNA expression of Nudt1 was statistically unaltered that likely resulted from higher variation of measurements. The accumulation of 8-oxodG with age is not a direct consequence of decreased enzyme activity toward singlet oxygen-induced substrate DNA. An age-related higher level of 8-oxodG even occurs concomitantly with high 8-oxodGTPase activity.

U2 - 10.1016/j.freeradbiomed.2009.05.030

DO - 10.1016/j.freeradbiomed.2009.05.030

M3 - Journal article

C2 - 19500668

VL - 47

SP - 608

EP - 615

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

IS - 5

ER -

ID: 14411395