Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture

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Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture. / Hansen, Sanni; Otten, Nina D.; Fjeldborg, Julie; Baptiste, Keith E.; Horohov, David W.

In: Experimental Gerontology, Vol. 124, 110634, 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, S, Otten, ND, Fjeldborg, J, Baptiste, KE & Horohov, DW 2019, 'Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture', Experimental Gerontology, vol. 124, 110634. https://doi.org/10.1016/j.exger.2019.110634

APA

Hansen, S., Otten, N. D., Fjeldborg, J., Baptiste, K. E., & Horohov, D. W. (2019). Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture. Experimental Gerontology, 124, [110634]. https://doi.org/10.1016/j.exger.2019.110634

Vancouver

Hansen S, Otten ND, Fjeldborg J, Baptiste KE, Horohov DW. Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture. Experimental Gerontology. 2019;124. 110634. https://doi.org/10.1016/j.exger.2019.110634

Author

Hansen, Sanni ; Otten, Nina D. ; Fjeldborg, Julie ; Baptiste, Keith E. ; Horohov, David W. / Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture. In: Experimental Gerontology. 2019 ; Vol. 124.

Bibtex

@article{cb17366cdedd4213abc4b3d62756214c,
title = "Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture",
abstract = "The objectives of this study were to evaluate the natural age-related variation and compare the level of pro-inflammatory cytokines in the peripheral blood and lower airways of horses. The mRNA expression of IL-1β, IL-6, IL-8 TNF-α TLR-4 in bronchoalveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMC) were studied by quantitative real time polymerase chain reaction (PCR) and differential cell count cytology from 44 horses of different ages. A significant age-related increase was found for the mRNA expression of IL-6, IL-8, TLR-4 and TNF-α in stimulated BAL cells and for TNF-α in stimulated PBMC. Furthermore, a significant decrease was found in the mRNA expression of IL-1β and TNF-α in stimulated BAL cells compared to stimulated PBMC. In conclusion, continued low antigen exposure of horses in the pasture environment could lead to a tight regulation of mRNA gene expression in the airway space compared to the peripheral blood and favour an age-related accumulation of mRNA genes.",
keywords = "BAL, Cytokine, Equine, Immunosenescence, Inflamm-aging",
author = "Sanni Hansen and Otten, {Nina D.} and Julie Fjeldborg and Baptiste, {Keith E.} and Horohov, {David W.}",
year = "2019",
doi = "10.1016/j.exger.2019.110634",
language = "English",
volume = "124",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Age-related dynamics of pro-inflammatory cytokines in equine bronchoalveolar lavage (BAL) fluid and peripheral blood from horses managed on pasture

AU - Hansen, Sanni

AU - Otten, Nina D.

AU - Fjeldborg, Julie

AU - Baptiste, Keith E.

AU - Horohov, David W.

PY - 2019

Y1 - 2019

N2 - The objectives of this study were to evaluate the natural age-related variation and compare the level of pro-inflammatory cytokines in the peripheral blood and lower airways of horses. The mRNA expression of IL-1β, IL-6, IL-8 TNF-α TLR-4 in bronchoalveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMC) were studied by quantitative real time polymerase chain reaction (PCR) and differential cell count cytology from 44 horses of different ages. A significant age-related increase was found for the mRNA expression of IL-6, IL-8, TLR-4 and TNF-α in stimulated BAL cells and for TNF-α in stimulated PBMC. Furthermore, a significant decrease was found in the mRNA expression of IL-1β and TNF-α in stimulated BAL cells compared to stimulated PBMC. In conclusion, continued low antigen exposure of horses in the pasture environment could lead to a tight regulation of mRNA gene expression in the airway space compared to the peripheral blood and favour an age-related accumulation of mRNA genes.

AB - The objectives of this study were to evaluate the natural age-related variation and compare the level of pro-inflammatory cytokines in the peripheral blood and lower airways of horses. The mRNA expression of IL-1β, IL-6, IL-8 TNF-α TLR-4 in bronchoalveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMC) were studied by quantitative real time polymerase chain reaction (PCR) and differential cell count cytology from 44 horses of different ages. A significant age-related increase was found for the mRNA expression of IL-6, IL-8, TLR-4 and TNF-α in stimulated BAL cells and for TNF-α in stimulated PBMC. Furthermore, a significant decrease was found in the mRNA expression of IL-1β and TNF-α in stimulated BAL cells compared to stimulated PBMC. In conclusion, continued low antigen exposure of horses in the pasture environment could lead to a tight regulation of mRNA gene expression in the airway space compared to the peripheral blood and favour an age-related accumulation of mRNA genes.

KW - BAL

KW - Cytokine

KW - Equine

KW - Immunosenescence

KW - Inflamm-aging

U2 - 10.1016/j.exger.2019.110634

DO - 10.1016/j.exger.2019.110634

M3 - Journal article

C2 - 31201919

AN - SCOPUS:85067276940

VL - 124

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

M1 - 110634

ER -

ID: 226536388