Achieving delayed release of freeze-dried probiotic strains by extrusion, spheronization and fluid bed coating - evaluated using a three-step in vitro model
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Achieving delayed release of freeze-dried probiotic strains by extrusion, spheronization and fluid bed coating - evaluated using a three-step in vitro model. / Jacobsen, Natashia Mai Yde; Caglayan, Ibrahim; Caglayan, Aslihan; Bar-Shalom, Daniel; Mullertz, Anette.
In: International Journal of Pharmaceutics, Vol. 591, 120022, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Achieving delayed release of freeze-dried probiotic strains by extrusion, spheronization and fluid bed coating - evaluated using a three-step in vitro model
AU - Jacobsen, Natashia Mai Yde
AU - Caglayan, Ibrahim
AU - Caglayan, Aslihan
AU - Bar-Shalom, Daniel
AU - Mullertz, Anette
PY - 2020
Y1 - 2020
N2 - Intake of probiotics is associated with many health benefits, which has generated an interest in formulating viable probiotic supplements. The present study had two aims. The first aim was to achieve gastrointestinal protection and delayed release of viable probiotics by pelletizing and coating freeze-dried probiotic strains, using riboflavin as a marker for release. The second aim was to set up a dynamic three-step in vitro model simulating the conditions in the human gastric, duodenum/jejunum and ileum compartments using physiologically relevant media to evaluate delayed release of the formulations. To simulate lowered bile acid concentrations in the ileum area of the gastrointestinal tract, a novel method using the bile acid sequestrant cholestyramine to lower bile acid concentrations in the small intestinal medium to physiologically relevant levels was attempted. Granulation, extrusion and spheronization was used to develop pellets containing viable probiotics using freeze-dried Lactobacullus reuteri as a model strain. Fluid bed coating the pellets with the pH-sensitive polymers Eudragit S100 or Eudragit FS30D resulted in targeted release in the ileum step of the three-step in vitro model based on release of the marker riboflavin.
AB - Intake of probiotics is associated with many health benefits, which has generated an interest in formulating viable probiotic supplements. The present study had two aims. The first aim was to achieve gastrointestinal protection and delayed release of viable probiotics by pelletizing and coating freeze-dried probiotic strains, using riboflavin as a marker for release. The second aim was to set up a dynamic three-step in vitro model simulating the conditions in the human gastric, duodenum/jejunum and ileum compartments using physiologically relevant media to evaluate delayed release of the formulations. To simulate lowered bile acid concentrations in the ileum area of the gastrointestinal tract, a novel method using the bile acid sequestrant cholestyramine to lower bile acid concentrations in the small intestinal medium to physiologically relevant levels was attempted. Granulation, extrusion and spheronization was used to develop pellets containing viable probiotics using freeze-dried Lactobacullus reuteri as a model strain. Fluid bed coating the pellets with the pH-sensitive polymers Eudragit S100 or Eudragit FS30D resulted in targeted release in the ileum step of the three-step in vitro model based on release of the marker riboflavin.
KW - In vitro model
KW - Targeted delivery
KW - Probiotics
KW - Extrusion-spheronization
KW - LACTOBACILLUS
KW - MICROENCAPSULATION
KW - SPHERONISATION
KW - FORMULATION
KW - VIABILITY
KW - LIPOLYSIS
KW - PRODUCTS
U2 - 10.1016/j.ijpharm.2020.120022
DO - 10.1016/j.ijpharm.2020.120022
M3 - Journal article
C2 - 33122110
VL - 591
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
M1 - 120022
ER -
ID: 255843333