A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes. / Jørgensen, Morten Egevang; Nour-Eldin, Hussam Hassan; Halkier, Barbara Ann.

Biotechnology of plant secondary metabolism: methods and protocols. ed. / Arthur Germano Fett-Neto. Springer, 2016. p. 99-107 (Methods in Molecular Biology, Vol. 1405).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Jørgensen, ME, Nour-Eldin, HH & Halkier, BA 2016, A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes. in AG Fett-Neto (ed.), Biotechnology of plant secondary metabolism: methods and protocols. Springer, Methods in Molecular Biology, vol. 1405, pp. 99-107. https://doi.org/10.1007/978-1-4939-3393-8_10

APA

Jørgensen, M. E., Nour-Eldin, H. H., & Halkier, B. A. (2016). A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes. In A. G. Fett-Neto (Ed.), Biotechnology of plant secondary metabolism: methods and protocols (pp. 99-107). Springer. Methods in Molecular Biology Vol. 1405 https://doi.org/10.1007/978-1-4939-3393-8_10

Vancouver

Jørgensen ME, Nour-Eldin HH, Halkier BA. A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes. In Fett-Neto AG, editor, Biotechnology of plant secondary metabolism: methods and protocols. Springer. 2016. p. 99-107. (Methods in Molecular Biology, Vol. 1405). https://doi.org/10.1007/978-1-4939-3393-8_10

Author

Jørgensen, Morten Egevang ; Nour-Eldin, Hussam Hassan ; Halkier, Barbara Ann. / A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes. Biotechnology of plant secondary metabolism: methods and protocols. editor / Arthur Germano Fett-Neto. Springer, 2016. pp. 99-107 (Methods in Molecular Biology, Vol. 1405).

Bibtex

@inbook{582661d2a73441c1845ee89767ed2731,
title = "A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes",
abstract = "Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.",
author = "J{\o}rgensen, {Morten Egevang} and Nour-Eldin, {Hussam Hassan} and Halkier, {Barbara Ann}",
year = "2016",
doi = "10.1007/978-1-4939-3393-8_10",
language = "English",
isbn = "978-1-4939-3391-4",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "99--107",
editor = "Fett-Neto, {Arthur Germano}",
booktitle = "Biotechnology of plant secondary metabolism",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes

AU - Jørgensen, Morten Egevang

AU - Nour-Eldin, Hussam Hassan

AU - Halkier, Barbara Ann

PY - 2016

Y1 - 2016

N2 - Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.

AB - Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.

U2 - 10.1007/978-1-4939-3393-8_10

DO - 10.1007/978-1-4939-3393-8_10

M3 - Book chapter

C2 - 26843169

SN - 978-1-4939-3391-4

T3 - Methods in Molecular Biology

SP - 99

EP - 107

BT - Biotechnology of plant secondary metabolism

A2 - Fett-Neto, Arthur Germano

PB - Springer

ER -

ID: 160972690