A porcine model of acute, haematogenous, localized osteomyelitis due to Staphylococcus aureus: a pathomorphological study
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A porcine model of acute, haematogenous, localized osteomyelitis due to Staphylococcus aureus : a pathomorphological study. / Johansen, Louise Kruse; Frees, Dorte; Aalbæk, Bent; Koch, Janne; Iburg, Tine Moesgaard; Nielsen, Ole Lerberg; Leifsson, Pall Skuli; Jensen, Henrik Michael Elvang.
In: APMIS : Acta pathologica, microbiologica et immunologica Scandinavica. Supplementum, Vol. 119, No. 2, 2011, p. 111-118.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A porcine model of acute, haematogenous, localized osteomyelitis due to Staphylococcus aureus
T2 - a pathomorphological study
AU - Johansen, Louise Kruse
AU - Frees, Dorte
AU - Aalbæk, Bent
AU - Koch, Janne
AU - Iburg, Tine Moesgaard
AU - Nielsen, Ole Lerberg
AU - Leifsson, Pall Skuli
AU - Jensen, Henrik Michael Elvang
PY - 2011
Y1 - 2011
N2 - A porcine model of acute, haematogenous, localized osteomyelitis was established. Serial dilutions of Staphylococcus aureus [5–50–500–5000–50 000 CFU/kg body weight (BW) suspended in saline or saline alone] were inoculated into the right brachial artery of pigs (BW 15 kg) separated into six groups of two animals. During the infection, blood was collected for cultivation, and after the animals were killed from day 5 to 15, they were necropsied and tissues were sampled for histopathology. Animals receiving =500 CFU/kg BW were free of lesions. Pigs inoculated with 5000 and 50 000 CFU/kg BW only developed microabscesses in bones of the infected legs. In the centre of microabscesses, S. aureus was regularly demonstrated together with necrotic neutrophils. Often, bone lesions resulted in trabecular osteonecrosis. The present localized model of acute haematogenous osteomyelitis revealed a pattern of development and presence of lesions similar to the situation in children. Therefore, this model should be reliably applied in studies of this disease with respect to e.g. pathophysiology and pathomorphology. Moreover, because of the regional containment of the infection to a defined number of bones, the model should be applicable also for screening of new therapy strategies.
AB - A porcine model of acute, haematogenous, localized osteomyelitis was established. Serial dilutions of Staphylococcus aureus [5–50–500–5000–50 000 CFU/kg body weight (BW) suspended in saline or saline alone] were inoculated into the right brachial artery of pigs (BW 15 kg) separated into six groups of two animals. During the infection, blood was collected for cultivation, and after the animals were killed from day 5 to 15, they were necropsied and tissues were sampled for histopathology. Animals receiving =500 CFU/kg BW were free of lesions. Pigs inoculated with 5000 and 50 000 CFU/kg BW only developed microabscesses in bones of the infected legs. In the centre of microabscesses, S. aureus was regularly demonstrated together with necrotic neutrophils. Often, bone lesions resulted in trabecular osteonecrosis. The present localized model of acute haematogenous osteomyelitis revealed a pattern of development and presence of lesions similar to the situation in children. Therefore, this model should be reliably applied in studies of this disease with respect to e.g. pathophysiology and pathomorphology. Moreover, because of the regional containment of the infection to a defined number of bones, the model should be applicable also for screening of new therapy strategies.
U2 - 10.1111/j.1600-0463.2010.02700.x
DO - 10.1111/j.1600-0463.2010.02700.x
M3 - Journal article
VL - 119
SP - 111
EP - 118
JO - APMIS. Supplementum
JF - APMIS. Supplementum
SN - 0903-465X
IS - 2
ER -
ID: 32174425