A High-Density Map for Navigating the Human Polycomb Complexome
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A High-Density Map for Navigating the Human Polycomb Complexome. / Hauri, Simon; Comoglio, Federico; Seimiya, Makiko; Gerstung, Moritz; Glatter, Timo; Hansen, Klaus; Aebersold, Ruedi; Paro, Renato; Gstaiger, Matthias; Beisel, Christian.
In: Cell Reports, Vol. 17, No. 2, 04.10.2016, p. 583-595.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A High-Density Map for Navigating the Human Polycomb Complexome
AU - Hauri, Simon
AU - Comoglio, Federico
AU - Seimiya, Makiko
AU - Gerstung, Moritz
AU - Glatter, Timo
AU - Hansen, Klaus
AU - Aebersold, Ruedi
AU - Paro, Renato
AU - Gstaiger, Matthias
AU - Beisel, Christian
N1 - Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2016/10/4
Y1 - 2016/10/4
N2 - Polycomb group (PcG) proteins are major determinants of gene silencing and epigenetic memory in higher eukaryotes. Here, we systematically mapped the human PcG complexome using a robust affinity purification mass spectrometry approach. Our high-density protein interaction network uncovered a diverse range of PcG complexes. Moreover, our analysis identified PcG interactors linking them to the PcG system, thus providing insight into the molecular function of PcG complexes and mechanisms of recruitment to target genes. We identified two human PRC2 complexes and two PR-DUB deubiquitination complexes, which contain the O-linked N-acetylglucosamine transferase OGT1 and several transcription factors. Finally, genome-wide profiling of PR-DUB components indicated that the human PR-DUB and PRC1 complexes bind distinct sets of target genes, suggesting differential impact on cellular processes in mammals.
AB - Polycomb group (PcG) proteins are major determinants of gene silencing and epigenetic memory in higher eukaryotes. Here, we systematically mapped the human PcG complexome using a robust affinity purification mass spectrometry approach. Our high-density protein interaction network uncovered a diverse range of PcG complexes. Moreover, our analysis identified PcG interactors linking them to the PcG system, thus providing insight into the molecular function of PcG complexes and mechanisms of recruitment to target genes. We identified two human PRC2 complexes and two PR-DUB deubiquitination complexes, which contain the O-linked N-acetylglucosamine transferase OGT1 and several transcription factors. Finally, genome-wide profiling of PR-DUB components indicated that the human PR-DUB and PRC1 complexes bind distinct sets of target genes, suggesting differential impact on cellular processes in mammals.
U2 - 10.1016/j.celrep.2016.08.096
DO - 10.1016/j.celrep.2016.08.096
M3 - Journal article
C2 - 27705803
VL - 17
SP - 583
EP - 595
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 2
ER -
ID: 168454120