A high-density genetic map and QTL mapping of leaf traits and glucosinolates in Barbarea vulgaris
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A high-density genetic map and QTL mapping of leaf traits and glucosinolates in Barbarea vulgaris. / Liu, Tong Jin; Zhang, You Jun; Agerbirk, Niels; Wang, Hai Ping; Wei, Xiao Chun; Song, Jiang Ping; He, Hong Ju; Zhao, Xue Zhi; Zhang, Xiao Hui; Li, Xi Xiang.
In: BMC Genomics, Vol. 20, No. 1, 371, 14.05.2019, p. 1-14.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A high-density genetic map and QTL mapping of leaf traits and glucosinolates in Barbarea vulgaris
AU - Liu, Tong Jin
AU - Zhang, You Jun
AU - Agerbirk, Niels
AU - Wang, Hai Ping
AU - Wei, Xiao Chun
AU - Song, Jiang Ping
AU - He, Hong Ju
AU - Zhao, Xue Zhi
AU - Zhang, Xiao Hui
AU - Li, Xi Xiang
PY - 2019/5/14
Y1 - 2019/5/14
N2 - Background: Barbarea vulgaris is a wild cruciferous plant and include two distinct types: the G- and P-types named after their glabrous and pubescent leaves, respectively. The types differ significantly in resistance to a range of insects and diseases as well as glucosinolates and other chemical defenses. A high-density linkage map was needed for further progress to be made in the molecular research of this plant. Results: We performed restriction site-associated DNA sequencing (RAD-Seq) on an F 2 population generated from G- and P-type B. vulgaris. A total of 1545 SNP markers were mapped and ordered in eight linkage groups, which represents the highest density linkage map to date for the crucifer tribe Cardamineae. A total of 722 previously published genome contigs (50.2 Mb, 30% of the total length) can be anchored to this high density genetic map, an improvement compared to a previously published map (431 anchored contigs, 38.7 Mb, 23% of the assembly genome). Most of these (572 contigs, 31.2 Mb) were newly anchored to the map, representing a significant improvement. On the basis of the present high-density genetic map, 37 QTL were detected for eleven traits, each QTL explaining 2.9-71.3% of the phenotype variation. QTL of glucosinolates, leaf size and color traits were in most cases overlapping, possibly implying a functional connection. Conclusions: This high-density linkage map and the QTL obtained in this study will be useful for further understanding of the genetic of the B. vulgaris and molecular basis of these traits, many of which are shared in the related crop watercress.
AB - Background: Barbarea vulgaris is a wild cruciferous plant and include two distinct types: the G- and P-types named after their glabrous and pubescent leaves, respectively. The types differ significantly in resistance to a range of insects and diseases as well as glucosinolates and other chemical defenses. A high-density linkage map was needed for further progress to be made in the molecular research of this plant. Results: We performed restriction site-associated DNA sequencing (RAD-Seq) on an F 2 population generated from G- and P-type B. vulgaris. A total of 1545 SNP markers were mapped and ordered in eight linkage groups, which represents the highest density linkage map to date for the crucifer tribe Cardamineae. A total of 722 previously published genome contigs (50.2 Mb, 30% of the total length) can be anchored to this high density genetic map, an improvement compared to a previously published map (431 anchored contigs, 38.7 Mb, 23% of the assembly genome). Most of these (572 contigs, 31.2 Mb) were newly anchored to the map, representing a significant improvement. On the basis of the present high-density genetic map, 37 QTL were detected for eleven traits, each QTL explaining 2.9-71.3% of the phenotype variation. QTL of glucosinolates, leaf size and color traits were in most cases overlapping, possibly implying a functional connection. Conclusions: This high-density linkage map and the QTL obtained in this study will be useful for further understanding of the genetic of the B. vulgaris and molecular basis of these traits, many of which are shared in the related crop watercress.
KW - Barbarea vulgaris
KW - Genetic linkage map
KW - Glucosinolates
KW - Leaf traits
KW - QTL
KW - Restriction site-associated DNA sequencing (RAD-Seq)
UR - http://www.scopus.com/inward/record.url?scp=85065720707&partnerID=8YFLogxK
U2 - 10.1186/s12864-019-5769-z
DO - 10.1186/s12864-019-5769-z
M3 - Journal article
C2 - 31088355
AN - SCOPUS:85065720707
VL - 20
SP - 1
EP - 14
JO - BMC Genomics
JF - BMC Genomics
SN - 1471-2164
IS - 1
M1 - 371
ER -
ID: 223821027