A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development

Research output: Contribution to journalJournal articlepeer-review

Standard

A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development. / Nielsen, J; Christiansen, J; Lykke-Andersen, J; Johnsen, A H; Wewer, Ulla M.; Nielsen, F C.

In: Molecular and Cellular Biology, Vol. 19, No. 2, 1999, p. 1262-1270.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Nielsen, J, Christiansen, J, Lykke-Andersen, J, Johnsen, AH, Wewer, UM & Nielsen, FC 1999, 'A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development', Molecular and Cellular Biology, vol. 19, no. 2, pp. 1262-1270. <http://mcb.asm.org/cgi/content/abstract/19/2/1262>

APA

Nielsen, J., Christiansen, J., Lykke-Andersen, J., Johnsen, A. H., Wewer, U. M., & Nielsen, F. C. (1999). A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development. Molecular and Cellular Biology, 19(2), 1262-1270. http://mcb.asm.org/cgi/content/abstract/19/2/1262

Vancouver

Nielsen J, Christiansen J, Lykke-Andersen J, Johnsen AH, Wewer UM, Nielsen FC. A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development. Molecular and Cellular Biology. 1999;19(2):1262-1270.

Author

Nielsen, J ; Christiansen, J ; Lykke-Andersen, J ; Johnsen, A H ; Wewer, Ulla M. ; Nielsen, F C. / A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development. In: Molecular and Cellular Biology. 1999 ; Vol. 19, No. 2. pp. 1262-1270.

Bibtex

@article{f9d41a7074c811dbbee902004c4f4f50,
title = "A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development",
abstract = "Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit multiple attachments to the 5' UTR from the translationally regulated IGF-II leader 3 mRNA but are unable to bind to the 5' UTR from the constitutively translated IGF-II leader 4 mRNA. IMPs contain the unique combination of two RNA recognition motifs and four hnRNP K homology domains and are homologous to the Xenopus Vera and chicken zipcode-binding proteins. IMP localizes to subcytoplasmic domains in a growth-dependent and cell-specific manner and causes a dose-dependent translational repression of IGF-II leader 3 -luciferase mRNA. Mouse IMPs are produced in a burst at embryonic day 12.5 followed by a decline towards birth, and, similar to IGF-II, IMPs are especially expressed in developing epithelia, muscle, and placenta in both mouse and human embryos. The results imply that cytoplasmic 5' UTR-binding proteins control IGF-II biosynthesis during late mammalian development.",
keywords = "3T3 Cells, Amino Acid Sequence, Animals, Binding Sites, Cell Line, Chickens, Cloning, Molecular, DNA, Complementary, Embryonic and Fetal Development, Gene Expression Regulation, Developmental, Humans, Insulin-Like Growth Factor II, Mice, Molecular Sequence Data, Protein Biosynthesis, RNA, Messenger, RNA-Binding Proteins, Sequence Homology, Amino Acid, Xenopus",
author = "J Nielsen and J Christiansen and J Lykke-Andersen and Johnsen, {A H} and Wewer, {Ulla M.} and Nielsen, {F C}",
year = "1999",
language = "English",
volume = "19",
pages = "1262--1270",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development

AU - Nielsen, J

AU - Christiansen, J

AU - Lykke-Andersen, J

AU - Johnsen, A H

AU - Wewer, Ulla M.

AU - Nielsen, F C

PY - 1999

Y1 - 1999

N2 - Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit multiple attachments to the 5' UTR from the translationally regulated IGF-II leader 3 mRNA but are unable to bind to the 5' UTR from the constitutively translated IGF-II leader 4 mRNA. IMPs contain the unique combination of two RNA recognition motifs and four hnRNP K homology domains and are homologous to the Xenopus Vera and chicken zipcode-binding proteins. IMP localizes to subcytoplasmic domains in a growth-dependent and cell-specific manner and causes a dose-dependent translational repression of IGF-II leader 3 -luciferase mRNA. Mouse IMPs are produced in a burst at embryonic day 12.5 followed by a decline towards birth, and, similar to IGF-II, IMPs are especially expressed in developing epithelia, muscle, and placenta in both mouse and human embryos. The results imply that cytoplasmic 5' UTR-binding proteins control IGF-II biosynthesis during late mammalian development.

AB - Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit multiple attachments to the 5' UTR from the translationally regulated IGF-II leader 3 mRNA but are unable to bind to the 5' UTR from the constitutively translated IGF-II leader 4 mRNA. IMPs contain the unique combination of two RNA recognition motifs and four hnRNP K homology domains and are homologous to the Xenopus Vera and chicken zipcode-binding proteins. IMP localizes to subcytoplasmic domains in a growth-dependent and cell-specific manner and causes a dose-dependent translational repression of IGF-II leader 3 -luciferase mRNA. Mouse IMPs are produced in a burst at embryonic day 12.5 followed by a decline towards birth, and, similar to IGF-II, IMPs are especially expressed in developing epithelia, muscle, and placenta in both mouse and human embryos. The results imply that cytoplasmic 5' UTR-binding proteins control IGF-II biosynthesis during late mammalian development.

KW - 3T3 Cells

KW - Amino Acid Sequence

KW - Animals

KW - Binding Sites

KW - Cell Line

KW - Chickens

KW - Cloning, Molecular

KW - DNA, Complementary

KW - Embryonic and Fetal Development

KW - Gene Expression Regulation, Developmental

KW - Humans

KW - Insulin-Like Growth Factor II

KW - Mice

KW - Molecular Sequence Data

KW - Protein Biosynthesis

KW - RNA, Messenger

KW - RNA-Binding Proteins

KW - Sequence Homology, Amino Acid

KW - Xenopus

M3 - Journal article

C2 - 9891060

VL - 19

SP - 1262

EP - 1270

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 2

ER -

ID: 191547