A comparison of protein kinases inhibitor screening methods using both enzymatic activity and binding affinity determination

Research output: Contribution to journalJournal articleResearchpeer-review

Binding assays are increasingly used as a screening method for protein kinase inhibitors; however, as yet only a weak correlation with enzymatic activity-based assays has been demonstrated. We show that the correlation between the two types of assays can be improved using more precise screening conditions. Furthermore a marked improvement in the correlation was found by using kinase constructs containing the catalytic domain in presence of additional domains or subunits.

Original languageEnglish
Article numbere98800
JournalPloS one
Issue number6
Pages (from-to)1-5
Number of pages6
Publication statusPublished - 2014

ID: 117611949