13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells. / Sonnewald, Ursula; Schousboe, Arne; Waagepetersen, Helle S.

Cell Culture Techniques. ed. / Michael Aschner; Cristina Sunol; Anna Bal-Price. 2011. p. 403-415 (Neuromethods, Vol. 56).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Sonnewald, U, Schousboe, A & Waagepetersen, HS 2011, 13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells. in M Aschner, C Sunol & A Bal-Price (eds), Cell Culture Techniques. Neuromethods, vol. 56, pp. 403-415. https://doi.org/10.1007/978-1-61779-077-5_20

APA

Sonnewald, U., Schousboe, A., & Waagepetersen, H. S. (2011). 13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells. In M. Aschner, C. Sunol, & A. Bal-Price (Eds.), Cell Culture Techniques (pp. 403-415). Neuromethods Vol. 56 https://doi.org/10.1007/978-1-61779-077-5_20

Vancouver

Sonnewald U, Schousboe A, Waagepetersen HS. 13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells. In Aschner M, Sunol C, Bal-Price A, editors, Cell Culture Techniques. 2011. p. 403-415. (Neuromethods, Vol. 56). https://doi.org/10.1007/978-1-61779-077-5_20

Author

Sonnewald, Ursula ; Schousboe, Arne ; Waagepetersen, Helle S. / 13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells. Cell Culture Techniques. editor / Michael Aschner ; Cristina Sunol ; Anna Bal-Price. 2011. pp. 403-415 (Neuromethods, Vol. 56).

Bibtex

@inbook{67474f9d732f4342bdb2203a25138f51,
title = "13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells",
abstract = "The use of 13C and 15N labeled precursors in combination with adequate analytical tools makes it possible to study metabolic pathways in cultured neural cells. The most commonly used precursors are 13C labeled glucose, lactate, glutamate and acetate. For a dynamic evaluation of intermediary metabolism of cell cultures, incubation with 13C containing substrates followed by nuclear magnetic resonance spectroscopy (NMRS) and mass spectrometry (MS) is excellent. NMRS can be used on cell extracts or living cells if a sufficient quantity of labeled atoms is present. MS is the more sensitive of the two methods but often it requires derivatization and separation of the components before analysis. The review provides descriptions of the basic and practical aspects of culturing neural cells, incubation and superfusion experiments and NMRS and MS analyses. It focuses on the analytical tools and the use of primary cultures of neurons and astrocytes for the elucidation of metabolic interactions between neurons and astrocytes.",
keywords = "[1-C]glucose, Cell culture, Mass spectrometry, Neurons, Nuclear magnetic resonance spectroscopy",
author = "Ursula Sonnewald and Arne Schousboe and Waagepetersen, {Helle S.}",
year = "2011",
month = mar,
day = "18",
doi = "10.1007/978-1-61779-077-5_20",
language = "English",
isbn = "9781617790768",
series = "Neuromethods",
publisher = "Humana Press",
pages = "403--415",
editor = "Michael Aschner and Cristina Sunol and Anna Bal-Price",
booktitle = "Cell Culture Techniques",

}

RIS

TY - CHAP

T1 - 13C NMR spectroscopy and mass spectrometry analysis of intermediary metabolism in cultured neural cells

AU - Sonnewald, Ursula

AU - Schousboe, Arne

AU - Waagepetersen, Helle S.

PY - 2011/3/18

Y1 - 2011/3/18

N2 - The use of 13C and 15N labeled precursors in combination with adequate analytical tools makes it possible to study metabolic pathways in cultured neural cells. The most commonly used precursors are 13C labeled glucose, lactate, glutamate and acetate. For a dynamic evaluation of intermediary metabolism of cell cultures, incubation with 13C containing substrates followed by nuclear magnetic resonance spectroscopy (NMRS) and mass spectrometry (MS) is excellent. NMRS can be used on cell extracts or living cells if a sufficient quantity of labeled atoms is present. MS is the more sensitive of the two methods but often it requires derivatization and separation of the components before analysis. The review provides descriptions of the basic and practical aspects of culturing neural cells, incubation and superfusion experiments and NMRS and MS analyses. It focuses on the analytical tools and the use of primary cultures of neurons and astrocytes for the elucidation of metabolic interactions between neurons and astrocytes.

AB - The use of 13C and 15N labeled precursors in combination with adequate analytical tools makes it possible to study metabolic pathways in cultured neural cells. The most commonly used precursors are 13C labeled glucose, lactate, glutamate and acetate. For a dynamic evaluation of intermediary metabolism of cell cultures, incubation with 13C containing substrates followed by nuclear magnetic resonance spectroscopy (NMRS) and mass spectrometry (MS) is excellent. NMRS can be used on cell extracts or living cells if a sufficient quantity of labeled atoms is present. MS is the more sensitive of the two methods but often it requires derivatization and separation of the components before analysis. The review provides descriptions of the basic and practical aspects of culturing neural cells, incubation and superfusion experiments and NMRS and MS analyses. It focuses on the analytical tools and the use of primary cultures of neurons and astrocytes for the elucidation of metabolic interactions between neurons and astrocytes.

KW - [1-C]glucose

KW - Cell culture

KW - Mass spectrometry

KW - Neurons

KW - Nuclear magnetic resonance spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=79952579144&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-077-5_20

DO - 10.1007/978-1-61779-077-5_20

M3 - Book chapter

AN - SCOPUS:79952579144

SN - 9781617790768

T3 - Neuromethods

SP - 403

EP - 415

BT - Cell Culture Techniques

A2 - Aschner, Michael

A2 - Sunol, Cristina

A2 - Bal-Price, Anna

ER -

ID: 222391559